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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Phosphorylation
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RecombinantRabMAb

Recombinant Anti-TLK1 antibody [EPR2886] - BSA and Azide free (ab247733)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-TLK1 antibody [EPR2886] - BSA and Azide free (ab247733)
  • Anti-TLK1 antibody [EPR2886] - BSA and Azide free (ab247733)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2886] to TLK1 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

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Protein
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Recombinant human TLK1 protein (ab125563)

View more associated products

Overview

  • Product name

    Anti-TLK1 antibody [EPR2886] - BSA and Azide free
    See all TLK1 primary antibodies
  • Description

    Rabbit monoclonal [EPR2886] to TLK1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247733 is the carrier-free version of ab108987 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab247733 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2886
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Phosphorylation
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases

Associated products

  • Alternative Versions

    • Anti-TLK1 antibody [EPR2886] (ab108987)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
  • Recombinant Protein

    • Recombinant human TLK1 protein (ab125563)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab247733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 86 kDa (predicted molecular weight: 87 kDa).
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 86 kDa (predicted molecular weight: 87 kDa).
  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function

      Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by faciliting the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10'.
    • Tissue specificity

      Widely expressed. Present in fetal placenta, liver, kidney and pancreas but not heart or skeletal muscle. Also found in adult cell lines. Isoform 3 is ubiquitously expressed in all tissues examined.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
      Contains 1 protein kinase domain.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q9UKI8 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 9874 Human
      • Omim: 608438 Human
      • SwissProt: Q9UKI8 Human
      • Unigene: 726093 Human
      • Unigene: 744917 Human
      • Alternative names

        • PKU beta antibody
        • PKU-beta antibody
        • serine threonine protein kinase antibody
        • Serine/threonine protein kinase tousled like 1 antibody
        • Serine/threonine-protein kinase tousled-like 1 antibody
        • SNARE protein kinase SNAK antibody
        • Tlk1 antibody
        • TLK1_HUMAN antibody
        • Tousled like kinase 1 antibody
        • Tousled-like kinase 1 antibody
        see all

      Images

      • Western blot - Anti-TLK1 antibody [EPR2886] - BSA and Azide free (ab247733)
        Western blot - Anti-TLK1 antibody [EPR2886] - BSA and Azide free (ab247733)
        All lanes : Anti-TLK1 antibody [EPR2886] (ab108987) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : A431 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

        Predicted band size: 87 kDa
        Observed band size: 86 kDa
        why is the actual band size different from the predicted?



        This data was developed using ab108987, the same antibody clone in a different buffer formulation.

         

      • Anti-TLK1 antibody [EPR2886] - BSA and Azide free (ab247733)
        Anti-TLK1 antibody [EPR2886] - BSA and Azide free (ab247733)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

    • References (0)

      Publishing research using ab247733? Please let us know so that we can cite the reference in this datasheet.

      ab247733 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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