Recombinant Anti-TLK1 antibody [EPR2886] - BSA and Azide free (ab247733)
![Western blot - Anti-TLK1 antibody [EPR2886] - BSA and Azide free (ab247733)](https://www.abcam.com/ps/products/247/ab247733/Images/ab247733-398298-anti-tlk1-antibody-epr2886-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-TLK1 antibody [EPR2886] - BSA and Azide free (ab247733)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2886] to TLK1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-TLK1 antibody [EPR2886] - BSA and Azide free
See all TLK1 primary antibodies -
Description
Rabbit monoclonal [EPR2886] to TLK1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247733 is the carrier-free version of ab108987 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247733 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR2886 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab247733 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 86 kDa (predicted molecular weight: 87 kDa).
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 86 kDa (predicted molecular weight: 87 kDa). |
Target
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Function
Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by faciliting the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10'. -
Tissue specificity
Widely expressed. Present in fetal placenta, liver, kidney and pancreas but not heart or skeletal muscle. Also found in adult cell lines. Isoform 3 is ubiquitously expressed in all tissues examined. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9874 Human
- Omim: 608438 Human
- SwissProt: Q9UKI8 Human
- Unigene: 726093 Human
- Unigene: 744917 Human
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Alternative names
- PKU beta antibody
- PKU-beta antibody
- serine threonine protein kinase antibody
see all
Images
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Western blot - Anti-TLK1 antibody [EPR2886] - BSA and Azide free (ab247733)All lanes : Anti-TLK1 antibody [EPR2886] (ab108987) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 87 kDa
Observed band size: 86 kDa why is the actual band size different from the predicted?This data was developed using ab108987, the same antibody clone in a different buffer formulation.
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![Anti-TLK1 antibody [EPR2886] - BSA and Azide free (ab247733)](https://www.abcam.com/ps/products/247/ab247733/Images/ab247733-2-benefits-of-recombinant-antibodies.png)
Anti-TLK1 antibody [EPR2886] - BSA and Azide free (ab247733)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab247733 has not yet been referenced specifically in any publications.
