Key features and details
- Rat monoclonal [6C2] to TLR2
- Suitable for: IP, ICC/IF, Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2b
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab11864 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IF: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
Before use in biological assays the product should be filter sterilised. Depending on the working concentration, it may need to be dialysed against tissue culture medium to remove the sodium azide added.
Is unsuitable for WB.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionCooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.
Tissue specificityHighly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.
Sequence similaritiesBelongs to the Toll-like receptor family.
Contains 14 LRR (leucine-rich) repeats.
Contains 1 TIR domain.
modificationsGlycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.
- Information by UniProt
- CD282 antibody
- CD282 antigen antibody
- TIL 4 antibody
ab11864 staining TLR2 in Mouse primary macrophage by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Acetone for 5 minutes at -20°C and blocked with 1% BSA for 60 minutes at 25°C. Samples were incubated with primary antibody (1/150 in PBS + 1% BSA) for 2 hours at 25°C. A Cy5®-conjugated Goat anti-rat IgG monoclonal (1/500) was used as the secondary antibody.
ab11864 has been referenced in 3 publications.
- Yang Q et al. CD157 Confers Host Resistance to Mycobacterium tuberculosis via TLR2-CD157-PKCzeta-Induced Reactive Oxygen Species Production. mBio 10:N/A (2019). PubMed: 31455656
- Wang M et al. Microbial hijacking of complement-toll-like receptor crosstalk. Sci Signal 3:ra11 (2010). PubMed: 20159852
- Nilsen N et al. Lipopolysaccharide and double-stranded RNA up-regulate toll-like receptor 2 independently of myeloid differentiation factor 88. J Biol Chem 279:39727-35 (2004). PubMed: 15190057