Overview

  • Product name

  • Description

    Rabbit polyclonal to TLR2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide within Human TLR2 aa 730-780 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    D AAILILLEPI EKKAIPQRFC KLRKIMNTKT YLEWPMDEAQ REGFWVNLRA


    Database link: O60603

  • Positive control

    • Rat ovary tissue; mouse spleen and Human A549 cells; rat brain and rat lung lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab213676 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/1000. Detects a band of approximately 84 kDa (predicted molecular weight: 89 kDa).
Flow Cyt 1/20 - 1/100.

Target

  • Function

    Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.
  • Tissue specificity

    Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.
  • Sequence similarities

    Belongs to the Toll-like receptor family.
    Contains 14 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • Post-translational
    modifications

    Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD282 antibody
    • CD282 antigen antibody
    • TIL 4 antibody
    • TIL4 antibody
    • TLR 2 antibody
    • TLR2 antibody
    • TLR2_HUMAN antibody
    • Toll like receptor 2 antibody
    • Toll like receptor 2 precursor antibody
    • Toll-like receptor 2 antibody
    • Toll/interleukin 1 receptor like 4 antibody
    • Toll/interleukin 1 receptor like protein 4 antibody
    • Toll/interleukin receptor like protein 4 antibody
    • Toll/interleukin-1 receptor-like protein 4 antibody
    see all

Images

  • All lanes : Anti-TLR2 antibody (ab213676) at 1/200 dilution

    Lane 1 : rat brain lysate
    Lane 2 : rat lung lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/3000 dilution

    Predicted band size: 89 kDa



    12% gel

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat ovary tissue labeling TLR2 with ab213676 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

  • Flow cytometric analysis of TLR2 in Human A549 cells using ab213676 at 1/20 for 30 minutes, followed by a PE conjugated secondary antibody (green), compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat ovary tissue labeling TLR2 with ab213676 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

  • Flow cytometric analysis of TLR2 in mouse spleen cells using ab213676 at 1/20 for 30 minutes, followed by a PE conjugated secondary antibody (green), compared to control cells (blue), secondary only (light blue), and isotype control (orange).

References

This product has been referenced in:

  • Chen X  et al. Activation of multiple Toll-like receptors serves different roles in sepsis-induced acute lung injury. Exp Ther Med 18:443-450 (2019). Read more (PubMed: 31258682) »
  • Liu L  et al. Over-expressed microRNA-181a reduces glomerular sclerosis and renal tubular epithelial injury in rats with chronic kidney disease via down-regulation of the TLR/NF-?B pathway by binding to CRY1. Mol Med 24:49 (2018). Read more (PubMed: 30241461) »
See all 2 Publications for this product

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