Recombinant
RabMAb

Recombinant Anti-TLR2 antibody [EPR20302-119] (ab209216)

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Overview

  • Product name

    Anti-TLR2 antibody [EPR20302-119]
    See all TLR2 primary antibodies

  • Description

    Rabbit monoclonal [EPR20302-119] to TLR2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment within Mouse TLR2 aa 1-350. The exact sequence is proprietary.
    Database link: Q9QUN7

  • Positive control

    • WB: RAW 264.7 whole cell lysate. IHC-P: Mouse liver and lung tissues. ICC/IF: RAW 264.7 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab209216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
IHC-P 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100.

Fixation with 4% PFA is not recommended as no staining was obtained.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function

      Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.

    • Tissue specificity

      Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.

    • Sequence similarities

      Belongs to the Toll-like receptor family.
      Contains 14 LRR (leucine-rich) repeats.
      Contains 1 TIR domain.

    • Post-translational
      modifications

      Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD282 antibody
        • CD282 antigen antibody
        • TIL 4 antibody
        • TIL4 antibody
        • TLR 2 antibody
        • TLR2 antibody
        • TLR2_HUMAN antibody
        • Toll like receptor 2 antibody
        • Toll like receptor 2 precursor antibody
        • Toll-like receptor 2 antibody
        • Toll/interleukin 1 receptor like 4 antibody
        • Toll/interleukin 1 receptor like protein 4 antibody
        • Toll/interleukin receptor like protein 4 antibody
        • Toll/interleukin-1 receptor-like protein 4 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-TLR2 antibody [EPR20302-119] (ab209216)
        Immunocytochemistry/ Immunofluorescence - Anti-TLR2 antibody [EPR20302-119] (ab209216)

        Immunofluorescent analysis of 100% methanol-fixed RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling TLR2 with ab209216 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in RAW 264.7 cells.

        The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

        Secondary antibody only control: Used PBS instead of primary antibody followed by anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

        Fixation with 4% PFA is not recommended as no staining was obtained.

         

      • Western blot - Anti-TLR2 antibody [EPR20302-119] (ab209216)
        Western blot - Anti-TLR2 antibody [EPR20302-119] (ab209216)
        Anti-TLR2 antibody [EPR20302-119] (ab209216) at 1/1000 dilution + RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

        Predicted band size: 89 kDa
        Observed band size: 89 kDa


        Exposure time: 26 seconds


        Blocking and dilution buffer: 5% NFDM/TBST.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR2 antibody [EPR20302-119] (ab209216)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR2 antibody [EPR20302-119] (ab209216)

        Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling TLR2 with ab209216 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on macrophages of mouse lung (PMID: 24371556). Counter stained with hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

        The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. The section was incubated with ab209216 for 10 minutes at 37℃.

        Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR2 antibody [EPR20302-119] (ab209216)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR2 antibody [EPR20302-119] (ab209216)

        Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling TLR2 with ab209216 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on Kuffer cells of mouse liver (PMID: 21966468). Counter stained with hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

        The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. The section was incubated with ab209216 for 10 minutes at 37℃.

        Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      References

      ab209216 has not yet been referenced specifically in any publications.

      Publishing research using ab209216? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      Immunohistochemistry (Frozen sections) abreview for Anti-TLR2 antibody [EPR20302-119]

       Inconclusive
      Abreviews
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (oral epithelium)
      Permeabilization
      Yes - triton x-100
      Specification
      oral epithelium
      Blocking step
      BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0.553µg/mL · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Mr. Amit Leibovich

      Verified customer

      Submitted Jul 12 2019

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