Overview

  • Product name

    Anti-TLR2 antibody [EPR20303]
    See all TLR2 primary antibodies
  • Description

    Rabbit monoclonal [EPR20303] to TLR2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Mouse TLR2 aa 300-600. The exact sequence is proprietary.
    Database link: Q9QUN7

  • Positive control

    • WB: Mouse and rat spleen lysates; RAW 264.7 whole cell lysates untreated and treated with 1 µg/ml lipopolysaccharides for 6 hours . IP: RAW 264.7 treated with 1 µg/ml LPS for 6 hours whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab209217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
IP 1/30.

Target

  • Function

    Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.
  • Tissue specificity

    Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.
  • Sequence similarities

    Belongs to the Toll-like receptor family.
    Contains 14 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • Post-translational
    modifications

    Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD282 antibody
    • CD282 antigen antibody
    • TIL 4 antibody
    • TIL4 antibody
    • TLR 2 antibody
    • TLR2 antibody
    • TLR2_HUMAN antibody
    • Toll like receptor 2 antibody
    • Toll like receptor 2 precursor antibody
    • Toll-like receptor 2 antibody
    • Toll/interleukin 1 receptor like 4 antibody
    • Toll/interleukin 1 receptor like protein 4 antibody
    • Toll/interleukin receptor like protein 4 antibody
    • Toll/interleukin-1 receptor-like protein 4 antibody
    see all

Images

  • All lanes : Anti-TLR2 antibody [EPR20303] (ab209217) at 1/1000 dilution

    Lane 1 : Mouse spleen lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Mouse heart lysate
    Lane 4 : Rat spleen lysate
    Lane 5 : Rat brain lysate
    Lane 6 : Rat heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 89 kDa
    Observed band size: 89 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    TLR2 is expressed in spleen but not in brain and heart according to the literature (PMID: 11777946).

  • All lanes : Anti-TLR2 antibody [EPR20303] (ab209217) at 1/10000 dilution

    Lane 1 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : RAW 264.7 whole cell lysate treated with 1 µg/ml lipopolysaccharides for 6 hours

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 89 kDa
    Observed band size: 89 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Expression of TLR2 in RAW 264.7 cells can be enhanced by LPS treatment according to the literature (PMID: 11067935).

  • TLR2 was immunoprecipitated from 0.35 mg of RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate, treated with 1 µg/ml LPS for 6 hours, with ab209217 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab209217 at 1/500 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: RAW 264.7 whole cell lysate treated with 1 µg/ml LPS for 6 hours, 10 µg (Input).

    Lane 2: ab209217 IP in RAW 264.7 whole cell lysate treated with 1 µg/ml LPS for 6 hours.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209217 in RAW 264.7 whole cell lysate treated with 1 µg/ml LPS for 6 hours.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

References

ab209217 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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