Overview

  • Product name
    Anti-TLR2 antibody [T2.5]
    See all TLR2 primary antibodies
  • Description
    Mouse monoclonal [T2.5] to TLR2
  • Host species
    Mouse
  • Specificity
    The endotoxin concentration is < 24 EU/mg, determined with HIT302 LAL Assay. Negative control: CHO cells.
  • Tested applications
    Suitable for: Functional Studies, ICC/IF, WB, IP, Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse TLR2.
    Database link: Q9QUN7

  • Positive control
    • Flow Cyt: RAW 264.7 cells. WB: Human lymph node tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab16894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Mice were injected i.p. with 1 mg ab16894, after 1 hour incubation mice were challenged; 5 µg/ml was added to cell culture.

ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. PubMed: 25404049
IP Use at an assay dependent concentration.

40 µg cleared protein was incubated with 2 µg ab16894 for 1 hour at 4 °C.

Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

4 x 104 leukocytes/ml were stained for 30 minutes at 4 °C.

 

IHC-Fr Use at an assay dependent concentration.

6 µm sections were fixed with acetone. Sections were blocked with goat serum and exposed overnight.

Target

  • Function
    Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.
  • Tissue specificity
    Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.
  • Sequence similarities
    Belongs to the Toll-like receptor family.
    Contains 14 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • Post-translational
    modifications
    Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD282 antibody
    • CD282 antigen antibody
    • TIL 4 antibody
    • TIL4 antibody
    • TLR 2 antibody
    • TLR2 antibody
    • TLR2_HUMAN antibody
    • Toll like receptor 2 antibody
    • Toll like receptor 2 precursor antibody
    • Toll-like receptor 2 antibody
    • Toll/interleukin 1 receptor like 4 antibody
    • Toll/interleukin 1 receptor like protein 4 antibody
    • Toll/interleukin receptor like protein 4 antibody
    • Toll/interleukin-1 receptor-like protein 4 antibody
    see all

Images

  • Detection of TLR2 in RAW cells. Red, black and blue line represent the isotype control, cells only and ab16894 at 10 μg/ml, respectively.

  • Effect of anti-TLR antibodies on TNF production in a whole blood model.

  • Anti-TLR2 antibody [T2.5] (ab16894) at 1/100 dilution + Human lymph node tissue lysate - total protein (ab29871) at 10 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Observed band size: 89 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 60 kDa, 65 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Nguyen CT  et al. Inhibitory effects of superoxide dismutase 3 on Propionibacterium acnes-induced skin inflammation. Sci Rep 8:4024 (2018). Read more (PubMed: 29507345) »
  • Song H  et al. Worm expulsion of Gymnophalloides seoi from C57BL/6 mice: role of metacercarial exosomes in upregulating TLR2 and MUC2 expression in intestinal tissues. Parasitol Res 117:3309-3314 (2018). Read more (PubMed: 30019213) »
See all 7 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (human monocytic cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
human monocytic cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Aug 31 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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