Anti-TLR2 antibody [T2.5] (ab16894)
Key features and details
- Mouse monoclonal [T2.5] to TLR2
- Suitable for: WB, Functional Studies, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-TLR2 antibody [T2.5]
See all TLR2 primary antibodies -
Description
Mouse monoclonal [T2.5] to TLR2 -
Host species
Mouse -
Specificity
The endotoxin concentration is < 24 EU/mg, determined with HIT302 LAL Assay. Negative control: CHO cells. -
Tested applications
Suitable for: WB, Functional Studies, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Mouse TLR2.
Database link: Q9QUN7 -
Positive control
- Flow Cyt: RAW 264.7 cells. WB: Human lymph node tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
0.2 µm filtered -
Clonality
Monoclonal -
Clone number
T2.5 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab16894 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 89 kDa.
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Functional Studies |
Use at an assay dependent concentration.
Mice were injected i.p. with 1 mg ab16894, after 1 hour incubation mice were challenged; 5 µg/ml was added to cell culture. |
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. 4 x 104 leukocytes/ml were stained for 30 minutes at 4 °C.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 89 kDa. |
Functional Studies
Use at an assay dependent concentration. Mice were injected i.p. with 1 mg ab16894, after 1 hour incubation mice were challenged; 5 µg/ml was added to cell culture. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. 4 x 104 leukocytes/ml were stained for 30 minutes at 4 °C.
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Target
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Function
Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6. -
Tissue specificity
Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 14 LRR (leucine-rich) repeats.
Contains 1 TIR domain. -
Post-translational
modificationsGlycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 7097 Human
- Entrez Gene: 24088 Mouse
- Omim: 603028 Human
- SwissProt: O60603 Human
- SwissProt: Q9QUN7 Mouse
- Unigene: 519033 Human
- Unigene: 87596 Mouse
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Alternative names
- CD282 antibody
- CD282 antigen antibody
- TIL 4 antibody
see all
Images
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Detection of TLR2 in RAW cells. Red, black and blue line represent the isotype control, cells only and ab16894 at 10 μg/ml, respectively.
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Effect of anti-TLR antibodies on TNF production in a whole blood model.
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Anti-TLR2 antibody [T2.5] (ab16894) at 1/100 dilution + Human lymph node tissue lysate - total protein (ab29871) at 10 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 89 kDa
Observed band size: 89 kDa
Additional bands at: 60 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Protocols
Datasheets and documents
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Datasheet download
References (29)
ab16894 has been referenced in 29 publications.
- Liu L et al. The transmembrane adapter SCIMP recruits tyrosine kinase Syk to phosphorylate Toll-like receptors to mediate selective inflammatory outputs. J Biol Chem 298:101857 (2022). PubMed: 35337798
- Nakamura K et al. Extracellular DJ-1 induces sterile inflammation in the ischemic brain. PLoS Biol 19:e3000939 (2021). PubMed: 34014921
- Matsumoto K et al. Platelet CXCL4 mediates neutrophil extracellular traps formation in ANCA-associated vasculitis. Sci Rep 11:222 (2021). PubMed: 33420306
- El-Obeid A et al. Herbal melanin induces interleukin-1β secretion and production by human THP-1 monocytes via Toll-like receptor 2 and p38 MAPK activation. Exp Ther Med 22:1081 (2021). PubMed: 34447474
- Liu Z et al. Paeoniflorin inhibits the macrophage-related rosacea-like inflammatory reaction through the suppressor of cytokine signaling 3-apoptosis signal-regulating kinase 1-p38 pathway. Medicine (Baltimore) 100:e23986 (2021). PubMed: 33545988