Product nameAnti-TLR2 antibody [TL2.1]
See all TLR2 primary antibodies
DescriptionMouse monoclonal [TL2.1] to TLR2
Tested applicationsSuitable for: ICC/IF, Flow Cyt, IP, Neutralising, WB, Functional Studiesmore details
Species reactivityReacts with: Dog, Human, Cynomolgus monkey, Rhesus monkey, Common marmoset
Recombinant human TLR2 produced by the CHO/CD14 reporter cell line.
- WB: THP1 + LPS. IHC-P: Human TLR2. ICC: Human U937 cells. Flow: Human peripheral blood mononuclear cells.
Ab9100 is a TLR2 function blocking antibody that is useful for studies on the role of TLR2 as a pattern recognition receptor in microbial products induced cytokine production by TLR2 bearing cells such as human pheriperal blood mononuclear cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferConstituents: PBS, 1% BSA
Concentration information loading...
PurityProtein G purified
Purification notes0.2 µm filtered
Primary antibody notesAb9100 is a TLR2 function blocking antibody that is useful for studies on the role of TLR2 as a pattern recognition receptor in microbial products induced cytokine production by TLR2 bearing cells such as human pheriperal blood mononuclear cells.
Our Abpromise guarantee covers the use of ab9100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 - 10 µg/ml.
Fix with PBS/2% formalin for 15 min at 4°C; permeabilize with acetone, 10 min, -20°C (see Flo et al J Leukoc Biol which used a conjugated form of the antibody).
|Flow Cyt||Use 2-3µg for 105 cells.
Untreated and 0.4 % formaldehyde fixed cells can be used; do not permeabilize the cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
|Neutralising||Use at an assay dependent concentration. See Lien et al.|
Abcam recommends using non-reducing conditions.
|Functional Studies||Use at an assay dependent concentration.|
FunctionCooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.
Tissue specificityHighly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.
Sequence similaritiesBelongs to the Toll-like receptor family.
Contains 14 LRR (leucine-rich) repeats.
Contains 1 TIR domain.
modificationsGlycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.
- Information by UniProt
- CD282 antibody
- CD282 antigen antibody
- TIL 4 antibody
TLR2 staining on human peripheral blood mononuclear cells by using ab9100. Green: CD3+-cells; Red: CD14+ cells
All lanes : Anti-TLR2 antibody [TL2.1] (ab9100) at 1 µg/ml
Lane 1 : THP1
Lane 2 : THP1 + LPS
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/50000 dilution
Performed under reducing conditions.
This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was ran at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked with 2% BSA for 60 minutes before being incubated with ab9100 (anti-TLR2 antibody; 1 ug/mL) for 18 hours at 4°C. Antibody binding was detected using ab97040 (HRP-labelled goat anti-mouse IgG) at 1:50,000 dilutions for 1 hour at room temperature.Visualisation was conducted using ECL development solution ab133406.
This gel was run under reducing conditions resulting in weak signal. We recommend customers to use non-reducing conditions.
ab9100 staining TLR2 in human U937 cells by Immunocytochemistry. Cells were fixed with paraformaldehyde and blocking with 2% BSA was done for 30 minutes at 270C. Samples were incubated with primary antibody (1/50) for 1 hour at 27°C. An Alexa Fluor®568-conjugated goat polyclonal to mouse IgG (H&L) was used at dilution at 1/50 as secondary antibody.
This product has been referenced in:
- Galoian K et al. Toll like receptors TLR1/2, TLR6 and MUC5B as binding interaction partners with cytostatic proline rich polypeptide 1 in human chondrosarcoma. Int J Oncol 52:139-154 (2018). Read more (PubMed: 29138803) »
- Moretti IF et al. Plasmatic membrane toll-like receptor expressions in human astrocytomas. PLoS One 13:e0199211 (2018). Read more (PubMed: 29912993) »