Anti-TLR2 antibody [TL2.1] (ab9100)

Mouse monoclonal TLR2 antibody [TL2.1]. Validated in WB, IP, FuncS, Neut, Flow Cyt, ICC/IF and tested in Dog, Human, Cynomolgus monkey, Rhesus monkey, Common marmoset. Cited in 21 publication(s).

Overview

  • Product name
    Anti-TLR2 antibody [TL2.1]
    See all TLR2 primary antibodies
  • Description
    Mouse monoclonal [TL2.1] to TLR2
  • Host species
    Mouse
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IP, Neutralising, WB, Functional Studiesmore details
  • Species reactivity
    Reacts with: Dog, Human, Cynomolgus monkey, Rhesus monkey, Common marmoset
  • Immunogen

    Recombinant human TLR2 produced by the CHO/CD14 reporter cell line.

  • Positive control
    • WB: THP1 + LPS. IHC-P: Human TLR2. ICC: Human U937 cells. Flow: Human peripheral blood mononuclear cells.
  • General notes

    Ab9100 is a TLR2 function blocking antibody that is useful for studies on the role of TLR2 as a pattern recognition receptor in microbial products induced cytokine production by TLR2 bearing cells such as human pheriperal blood mononuclear cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab9100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 - 10 µg/ml.

Fix with PBS/2% formalin for 15 min at 4°C; permeabilize with acetone, 10 min, -20°C (see Flo et al J Leukoc Biol which used a conjugated form of the antibody).

Flow Cyt Use 2-3µg for 105 cells.

Untreated and 0.4 % formaldehyde fixed cells can be used; do not permeabilize the cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

IP Use at an assay dependent concentration.
Neutralising Use at an assay dependent concentration. See Lien et al.
WB 1/50.

Abcam recommends using non-reducing conditions.

Functional Studies Use at an assay dependent concentration.

Target

  • Function
    Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.
  • Tissue specificity
    Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.
  • Sequence similarities
    Belongs to the Toll-like receptor family.
    Contains 14 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • Post-translational
    modifications
    Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD282 antibody
    • CD282 antigen antibody
    • TIL 4 antibody
    • TIL4 antibody
    • TLR 2 antibody
    • TLR2 antibody
    • TLR2_HUMAN antibody
    • Toll like receptor 2 antibody
    • Toll like receptor 2 precursor antibody
    • Toll-like receptor 2 antibody
    • Toll/interleukin 1 receptor like 4 antibody
    • Toll/interleukin 1 receptor like protein 4 antibody
    • Toll/interleukin receptor like protein 4 antibody
    • Toll/interleukin-1 receptor-like protein 4 antibody
    see all

Images

  • TLR2 staining on human peripheral blood mononuclear cells by using ab9100. Green: CD3+-cells; Red: CD14+ cells
  • All lanes : Anti-TLR2 antibody [TL2.1] (ab9100) at 1 µg/ml

    Lane 1 : THP1
    Lane 2 : THP1 + LPS

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/50000 dilution

    Performed under reducing conditions.


    This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was ran at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked with 2% BSA for 60 minutes before being incubated with ab9100 (anti-TLR2 antibody; 1 ug/mL) for 18 hours at 4°C. Antibody binding was detected using ab97040 (HRP-labelled goat anti-mouse IgG)  at  1:50,000 dilutions for 1 hour at room temperature.Visualisation was conducted using ECL development solution ab133406.

    This gel was run under reducing conditions resulting in weak signal. We recommend customers to use non-reducing conditions.

  • ab9100 staining TLR2 in human U937 cells by Immunocytochemistry. Cells were fixed with paraformaldehyde and blocking with 2% BSA was done for 30 minutes at 270C. Samples were incubated with primary antibody (1/50) for 1 hour at 27°C. An Alexa Fluor®568-conjugated goat polyclonal to mouse IgG (H&L) was used at dilution at 1/50 as secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Galoian K  et al. Toll like receptors TLR1/2, TLR6 and MUC5B as binding interaction partners with cytostatic proline rich polypeptide 1 in human chondrosarcoma. Int J Oncol 52:139-154 (2018). Read more (PubMed: 29138803) »
  • Moretti IF  et al. Plasmatic membrane toll-like receptor expressions in human astrocytomas. PLoS One 13:e0199211 (2018). Read more (PubMed: 29912993) »
See all 21 Publications for this product

Customer reviews and Q&As

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1-7 of 7 Abreviews

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (macrophage)
Specification
macrophage
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 12 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Pig Cell (freshly isolated PBMC)
Permeabilization
No
Gating Strategy
living singlets
Specification
freshly isolated PBMC

Korneel Grauwet

Verified customer

Submitted Aug 01 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rabbit Tissue lysate - whole (Brain)
Loading amount
25 µg
Specification
Brain
Gel Running Conditions
Reduced Non-Denaturing (Native) (4-20% gradient)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Apr 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human Normal Tonsil)
Specification
Human Normal Tonsil
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: DAKO Flex High pH-PTLink
Permeabilization
No
Blocking step
(agent) for 5 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 27 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (pancreatic)
Specification
pancreatic
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: DAKO antigen retrieval buffer pH 6
Permeabilization
No

Dr. Ilka Werner-Martini

Verified customer

Submitted Nov 04 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Platelet Lysate (precleared))
Loading amount
25 µg
Specification
Platelet Lysate (precleared)
Gel Running Conditions
Reduced Denaturing
Blocking step
Thermo Starting Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C

Dr. Mahesh Shivananjappa

Verified customer

Submitted Jan 08 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (U937)
Specification
U937
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 27°C

Dr. Mahesh Shivananjappa

Verified customer

Submitted Oct 05 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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