PE Anti-TLR3 antibody [40C1285.6] (ab45093)
Key features and details
- PE Mouse monoclonal [40C1285.6] to TLR3
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Overview
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Product name
PE Anti-TLR3 antibody [40C1285.6]
See all TLR3 primary antibodies -
Description
PE Mouse monoclonal [40C1285.6] to TLR3 -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
This antibody was developed against KLH-conjugated synthetic peptide of human TLR3.
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Positive control
- Human intestine or Ramos. Flow Cyt (Intra): Ramos, A549 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
40C1285.6 -
Isotype
IgG1 -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab45093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
Notes |
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WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 104 kDa.
This antibody has tested negative by western blot in mouse intestine cell lysate.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
Tissue specificity
Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons. -
Involvement in disease
Defects in TLR3 are associated with herpes simplex encephalitis type 2 (HSE2) [MIM:613002]. HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. Note=TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 22 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Contains 1 TIR domain. -
Domain
ds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18. -
Post-translational
modificationsHeavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding. -
Cellular localization
Endoplasmic reticulum membrane. Endosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 7098 Human
- Omim: 603029 Human
- SwissProt: O15455 Human
- Unigene: 657724 Human
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Alternative names
- CD283 antibody
- CD283 antigen antibody
- IIAE2 antibody
see all
Images
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ab45093 staining TLR3 in Human A549 (lung epithelial) cell line by Flow Cytometry. The sample was incubated with the primary antibody (5μg/ml in 0.1% Saponin + 1% FCS in PBS) for 45 minutes at 4°C. 1 = isotype control, 2 = ab45093
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ab45093, at 3 ug/ 1X10^6 cells, staining Ramos cells by Intracellular flow cytometric analysis. Toll-Like-Receptor3 (open peak) and isotype negative control (shaded peak).
Datasheets and documents
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Datasheet download
References (1)
ab45093 has been referenced in 1 publication.
- Franzen-Röhl E et al. Increased cell-mediated immune responses in patients with recurrent herpes simplex virus type 2 meningitis. Clin Vaccine Immunol 18:655-60 (2011). Flow Cyt . PubMed: 21325490