Overview

  • Product name

  • Description

    Rabbit polyclonal to TLR3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Sheep, Human
    Predicted to work with: Cow, Cat
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 464-647 of Human TLR3.

  • Positive control

    • K562 and THP-1 whole cell lysates; HeLa cells; Human TOV-112D xenograft.

Properties

Applications

Our Abpromise guarantee covers the use of ab137722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)
WB 1/1000 - 1/10000. Predicted molecular weight: 104 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificity

    Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons.
  • Involvement in disease

    Defects in TLR3 are associated with herpes simplex encephalitis type 2 (HSE2) [MIM:613002]. HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. Note=TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system.
  • Sequence similarities

    Belongs to the Toll-like receptor family.
    Contains 22 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
    Contains 1 TIR domain.
  • Domain

    ds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18.
  • Post-translational
    modifications

    Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding.
  • Cellular localization

    Endoplasmic reticulum membrane. Endosome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD283 antibody
    • CD283 antigen antibody
    • IIAE2 antibody
    • TLR 3 antibody
    • Tlr3 antibody
    • TLR3_HUMAN antibody
    • Toll Like Receptor 3 antibody
    • Toll-like receptor 3 antibody
    see all

Images

  • All lanes : Anti-TLR3 antibody (ab137722) at 1/5000 dilution

    Lane 1 : K562 whole cell lysate
    Lane 2 : THP-1 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 104 kDa



    5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded TOV-112D xenograft labelling TLR3 with ab137722 at 1/100 dilution.
  • Immunofluorescenct analysis of methanol-fixed HeLa cells labelling TLR3 with ab137722 at 1/200 dilution. Lower image is co-stained with Hoechst 33342.

References

This product has been referenced in:

  • Lee EY  et al. Crystallinity of Double-Stranded RNA-Antimicrobial Peptide Complexes Modulates Toll-Like Receptor 3-Mediated Inflammation. ACS Nano 11:12145-12155 (2017). Read more (PubMed: 29016111) »
  • Yu L  et al. Functional Roles of Pattern Recognition Receptors That Recognize Virus Nucleic Acids in Human Adipose-Derived Mesenchymal Stem Cells. Biomed Res Int 2016:9872138 (2016). Read more (PubMed: 28105439) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10%
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA
Sample
Sheep Tissue sections (Brain tissue)
Specification
Brain tissue
Permeabilization
No
Fixative
Paraformaldehyde

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Verified customer

Submitted Jun 20 2014

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