Anti-TLR3 antibody (ab137722)
Key features and details
- Rabbit polyclonal to TLR3
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-TLR3 antibody
See all TLR3 primary antibodies -
Description
Rabbit polyclonal to TLR3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Cat -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 464-647 of Human TLR3.
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Positive control
- WB: K562 and THP-1 whole cell lysates; IHC-P: Human TOV-112D xenograft, mouse liver, rat pancreas tissue; IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137722 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 104 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Predicted molecular weight: 104 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
Tissue specificity
Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons. -
Involvement in disease
Defects in TLR3 are associated with herpes simplex encephalitis type 2 (HSE2) [MIM:613002]. HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. Note=TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 22 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Contains 1 TIR domain. -
Domain
ds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18. -
Post-translational
modificationsHeavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding. -
Cellular localization
Endoplasmic reticulum membrane. Endosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 493661 Cat
- Entrez Gene: 281535 Cow
- Entrez Gene: 7098 Human
- Entrez Gene: 142980 Mouse
- Omim: 603029 Human
- SwissProt: Q5TJ59 Cow
- SwissProt: O15455 Human
- SwissProt: Q99MB1 Mouse
see all -
Alternative names
- CD283 antibody
- CD283 antigen antibody
- IIAE2 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded TOV-112D xenograft labelling TLR3 with ab137722 at 1/100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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All lanes : Anti-TLR3 antibody (ab137722) at 1/5000 dilution
Lane 1 : K562 whole cell lysate
Lane 2 : THP-1 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 104 kDa
5% SDS PAGE -
Immunofluorescenct analysis of methanol-fixed HeLa cells labelling TLR3 with ab137722 at 1/200 dilution. Lower image is co-stained with Hoechst 33342.
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Immunohistochemical analysis of paraffin-embedded mouse liver tissue labelling TLR3 at cytoplasm with ab137722 at 1/1000 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labelling TLR3 at cytoplasm with ab137722 at 1/1000 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab137722 has been referenced in 3 publications.
- Cen X et al. Small molecule SMU-CX24 targeting toll-like receptor 3 counteracts inflammation: A novel approach to atherosclerosis therapy. Acta Pharm Sin B 12:3667-3681 (2022). PubMed: 36176917
- Lee EY et al. Crystallinity of Double-Stranded RNA-Antimicrobial Peptide Complexes Modulates Toll-Like Receptor 3-Mediated Inflammation. ACS Nano 11:12145-12155 (2017). PubMed: 29016111
- Yu L et al. Functional Roles of Pattern Recognition Receptors That Recognize Virus Nucleic Acids in Human Adipose-Derived Mesenchymal Stem Cells. Biomed Res Int 2016:9872138 (2016). PubMed: 28105439