Overview

  • Product name

  • Description

    Rabbit polyclonal to TLR3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    A peptide corresponding to 15 amino acids near the amino terminus of human TLR3

  • Positive control

    • ICC/IF: K562 cells. WB: EL4 and K562 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab62567 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 10 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 104 kDa).
ICC/IF Use a concentration of 2 µg/ml.

Target

  • Function

    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificity

    Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons.
  • Involvement in disease

    Defects in TLR3 are associated with herpes simplex encephalitis type 2 (HSE2) [MIM:613002]. HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. Note=TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system.
  • Sequence similarities

    Belongs to the Toll-like receptor family.
    Contains 22 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
    Contains 1 TIR domain.
  • Domain

    ds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18.
  • Post-translational
    modifications

    Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding.
  • Cellular localization

    Endoplasmic reticulum membrane. Endosome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD283 antibody
    • CD283 antigen antibody
    • IIAE2 antibody
    • TLR 3 antibody
    • Tlr3 antibody
    • TLR3_HUMAN antibody
    • Toll Like Receptor 3 antibody
    • Toll-like receptor 3 antibody
    see all

Images

  • Anti-TLR3 antibody (ab62567) at 0.5 µg/ml + EL4 cell lysate

    Predicted band size: 104 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?

  • Lane 1 : Anti-TLR3 antibody (ab62567) at 0.5 µg/ml
    Lane 2 : Anti-TLR3 antibody (ab62567) at 1 µg/ml
    Lane 3 : Anti-TLR3 antibody (ab62567) at 2 µg/ml

    All lanes : K562 cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 104 kDa
    Observed band size: 90 kDa why is the actual band size different from the predicted?



    There are multiple additional bands. We are unsure as to the identity of these extra bands.
  • Immunofluorescence of TLR3 in K562 cells using ab62567 at 2 ug/ml.

  • ab62567, at 10µg/ml, staining TLR3 in K562 cells by immunocytochemistry.

References

ab62567 has not yet been referenced specifically in any publications.

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