Overview

  • Product name
    Anti-TLR3 antibody [TLR3.7]
    See all TLR3 primary antibodies
  • Description
    Mouse monoclonal [TLR3.7] to TLR3
  • Host species
    Mouse
  • Specificity
    Ab12085 recognises TLR3.
  • Tested applications
    Suitable for: Inhibition Assay, Functional Studies, IHC-P, IP, Flow Cyt, ICC/IFmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Dog, Human, Pig
  • Immunogen

    Human Flag-tagged TLR3 stably expressed by Ba/F3 cells.

  • Positive control
    • Placenta and pancreas. Monocyte-derived immature dendritic cells (see paper by Matsumoto et al,2003). Monocytes, granulocytes, lymphocytes, human fibroblast, MRC-5 & FS-4 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab12085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Inhibition Assay Use at an assay dependent concentration. Use ligand poly(I):poly(C).
Functional Studies Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 22023039
IP Use at an assay dependent concentration. See paper by Matsumoto et al, 2003.
Flow Cyt 1/10.

See paper by Matsumoto et al, 2003.

 

 

 

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use at an assay dependent concentration. PubMed: 19656899
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
    • Tissue specificity
      Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons.
    • Involvement in disease
      Defects in TLR3 are associated with herpes simplex encephalitis type 2 (HSE2) [MIM:613002]. HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. Note=TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system.
    • Sequence similarities
      Belongs to the Toll-like receptor family.
      Contains 22 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
      Contains 1 LRRNT domain.
      Contains 1 TIR domain.
    • Domain
      ds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18.
    • Post-translational
      modifications
      Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding.
    • Cellular localization
      Endoplasmic reticulum membrane. Endosome membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD283 antibody
      • CD283 antigen antibody
      • IIAE2 antibody
      • TLR 3 antibody
      • Tlr3 antibody
      • TLR3_HUMAN antibody
      • Toll Like Receptor 3 antibody
      • Toll-like receptor 3 antibody
      see all

    Images

    • Flow cytometric detection of human TLR3 on THP-1 cells. Red line represents the isotype control and the black line represents ab12085 at 10 μg/ml.

    References

    This product has been referenced in:
    • Hwang SH  et al. Characteristics of Human Turbinate-Derived Mesenchymal Stem Cells Are Not Affected by Allergic Condition of Donor. PLoS One 10:e0138041 (2015). Read more (PubMed: 26376485) »
    • Yuan MM  et al. TLR3 expression correlates with apoptosis, proliferation and angiogenesis in hepatocellular carcinoma and predicts prognosis. BMC Cancer 15:245 (2015). IHC . Read more (PubMed: 25884709) »
    See all 10 Publications for this product

    Customer reviews and Q&As

    1-9 of 9 Q&A

    Answer

    I heard back from our lab that the most recent lot of ab12085 had an endotoxin level of 0.02EU/ml. The ab17264 has not been tested for endotoxin.

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    Answer



    I can confirm that IgG1 has a mass of ca 150kDa.

    https://www.abcam.com/index.html?pageconfig=resource&rid=11258&pid=10996

    This should not change very much between different mouse IgG1 (unconjugated) antibodies.

    ab12085 has not been specifically tested for its molecular weight and therefore we assume that it is in line with being a mouse IgG1 and therefore ca 150kDa.

    If you are looking at a gel of this product, I would like to note that it also contains BSA.

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    Answer

    Thank you for contacting us.


    The biological activity of the antibody has been described in rthe following reference from datasheet:

    Matsumoto, M et al; Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signalling. Biochem Biophys Res Commun 2002, 293: 1364


    Monoclonal antibody TLR3.7 inhibits dsRNA-induces IFN-beta production. 7.5*10E4 MRC5 cells were pre-treated with 10-20 µg/ml for 1-24h at 37°C.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Answer

    Thank you for contacting us.


    ab12085 can be used to inhibit the function of TLR3. The following publication describe the use of this clone in an inhibitory assay:

    Matsumoto, M et al; Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signalling. Biochem Biophys Res Commun 2002, 293: 1364.

    PMID:12054664

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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    Answer

    Thank you for contacting us regarding ab65355, cAMP Direct Immunoassay kit. As requested I have created a credit note for the original purchase. Your credit note ID is . I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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    Answer

    Thank you for contacting us. Unfortunately Anti-TLR3 antibody [TLR3.7] (ab12085) has not been tested for neutralising activity in rat. More information on the antibodies functional activity can be found from the following reference: Matsumoto M et al. Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling. Biochem Biophys Res Commun 293:1364-9 (2002). PubMed: 12054664 The antibody is known to bind the extracellular domain of TRL3. As this antibody is directed against human TRL3 the homology of this region with that of Rat needs to be taken into consideration when predicting if the antibody is likely to bind the rat protein. Through sequence comparison these two regions share 78% homology. We usually predict homologies of 85% and above to react. I can therefore not recommend using this antibody with rat samples. We only have two antibodies, ab62566 and ab117353 which have been used with rat protein but neither has been tested for their neutralising behaviour. I'm sorry I could not be of more help. If you need any further information please do not hesitate to ask.

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    Question
    Answer

    Thank you for contacting us. The following reference describes the development of the TLR3.7 clone and its use in an inhibition assay: Matsumoto, M et al; Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signalling. Biochem Biophys Res Commun 2002, 293: 1364. PMID:12054664 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Answer

    以外と製造元の返事が早かったです。 この回答は電話で申し上げにくいかと思いますので、メールで送った方がよいではないかと思います。 ***製造元よりの情報*** 中和(inhibition)の詳細は下記の文献に載ってありますので、ご参考いただけますようお願いいたします。 1) Matsumoto, M et al; Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signalling. Biochem Biophys Res Commun 2002, 293: 1364 Antibody blocking and IFN-b assays. MRC-5 cells in 24-well plates (7.5*104) were pre-treated with 20 lg/ml of anti-TLR2 mAb (TLR2.45), or anti-TLR3 mAb (TLR3.7) for 1 h at 37 °C, then stimulated with polymyxin B-treated poly(I):poly(C) (5, 10 lg/ml) for 24 h. The concentrations of IFN-b in the culture supernatants were measured by ELISA (TFB Inc.). 2) Matsumoto, M et al; Subcellular localization of Toll-like receptor 3 in human dendritic cells. J Immunol 2003, 171: 3154 そして、マウスへの使用に関しては実際テストされたことがございません。万が一、実験で期待通りの結果を得られなかった場合、保証の提供ができかねます。しかし、そうはいっても、必ずしも結果が得られない訳でもございません。 ***ここまで*** マウスにテストされていないので、Abreview 100% ディスカウントを案内してもよいと思います。もしお客様が興味がありましたら、私からご案内します。その際、メールアドレスさえをご連絡いただけると大変助かります。

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    Answer

    Thank you for contacting us for technical support regarding ab12085. This antibody has not been tested against other species than human and I would recommend running in parallel with your samples the positive controls of human placenta or pancreas lysate. There is a 80% homology between the human form and the mouse form of TLR3, so although this is not low as the antibody is a monoclonal antibody it will only recognise one epitope and if this epitope is not homologous in the human and rat and mouse the antibody will fail to cross react with your samples. I am in touch with the laboratory to try to find out other experimental details which should help, such as the lysis buffer to make sure the protein is well extracted, I'm sorry for the delay and appreciate your patience,

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