• Product name
    Anti-TLR3 antibody [TLR3.7] (Biotin)
    See all TLR3 primary antibodies
  • Description
    Mouse monoclonal [TLR3.7] to TLR3 (Biotin)
  • Host species
  • Conjugation
  • Tested applications
    Suitable for: Flow Cyt, WB, IHC-Fr, Functional Studies, ICC/IF, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Dog, Human
  • Immunogen

    Human Flag-tagged TLR3 stably expressed by Ba/F3 cells.

  • Positive control
    • Monocytes, granulocytes, lymphocytes, human fibroblast, MRC-5 & FS-4 cells.



Our Abpromise guarantee covers the use of ab17264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50.

ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


WB 1/50. Predicted molecular weight: 100 kDa.
IHC-Fr 1/50.
Functional Studies Use at an assay dependent concentration.  Monoclonal antibody TLR3.7 inhibits dsRNA-induces IFN-beta production
ICC/IF 1/50.
IP 1/50.
IHC-P 1/50.


  • Function
    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificity
    Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons.
  • Involvement in disease
    Defects in TLR3 are associated with herpes simplex encephalitis type 2 (HSE2) [MIM:613002]. HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. Note=TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system.
  • Sequence similarities
    Belongs to the Toll-like receptor family.
    Contains 22 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
    Contains 1 TIR domain.
  • Domain
    ds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18.
  • Post-translational
    Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding.
  • Cellular localization
    Endoplasmic reticulum membrane. Endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD283 antibody
    • CD283 antigen antibody
    • IIAE2 antibody
    • TLR 3 antibody
    • Tlr3 antibody
    • TLR3_HUMAN antibody
    • Toll Like Receptor 3 antibody
    • Toll-like receptor 3 antibody
    see all


  • Flow cytometric detection of human TLR3 on THP-1 cells. Red line represents the isotype control and the black line represents ab17264 at 10 μg/ml.


This product has been referenced in:
  • Matsumoto M  et al. Subcellular localization of Toll-like receptor 3 in human dendritic cells. J Immunol 171:3154-62 (2003). ICC/IF, Flow Cyt, IP ; Human . Read more (PubMed: 12960343) »
  • Matsumoto M  et al. Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling. Biochem Biophys Res Commun 293:1364-9 (2002). Read more (PubMed: 12054664) »

See all 2 Publications for this product

Customer reviews and Q&As

I heard back from our lab that the most recent lot of ab12085 had an endotoxin level of 0.02EU/ml. The ab17264 has not been tested for endotoxin.


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