Key features and details
- Mouse monoclonal [TLR3.7] to TLR3 (Biotin)
- Suitable for: Flow Cyt, WB, IHC-Fr, Functional Studies, ICC/IF, IP, IHC-P
- Reacts with: Mouse, Dog, Human
- Conjugation: Biotin
- Isotype: IgG1
Product nameAnti-TLR3 antibody [TLR3.7] (Biotin)
See all TLR3 primary antibodies
DescriptionMouse monoclonal [TLR3.7] to TLR3 (Biotin)
Tested applicationsSuitable for: Flow Cyt, WB, IHC-Fr, Functional Studies, ICC/IF, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Dog, Human
Human Flag-tagged TLR3 stably expressed by Ba/F3 cells.
- Monocytes, granulocytes, lymphocytes, human fibroblast, MRC-5 & FS-4 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA
Concentration information loading...
PurityProtein G purified
Purification notes0.2 µm filtered
Our Abpromise guarantee covers the use of ab17264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||1/50. Predicted molecular weight: 100 kDa.|
|Functional Studies||Use at an assay dependent concentration. Monoclonal antibody TLR3.7 inhibits dsRNA-induces IFN-beta production|
FunctionKey component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
Tissue specificityExpressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons.
Involvement in diseaseDefects in TLR3 are associated with herpes simplex encephalitis type 2 (HSE2) [MIM:613002]. HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. Note=TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system.
Sequence similaritiesBelongs to the Toll-like receptor family.
Contains 22 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Contains 1 TIR domain.
Domainds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18.
modificationsHeavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding.
Cellular localizationEndoplasmic reticulum membrane. Endosome membrane.
- Information by UniProt
- CD283 antibody
- CD283 antigen antibody
- IIAE2 antibody
ab17264 has been referenced in 2 publications.
- Matsumoto M et al. Subcellular localization of Toll-like receptor 3 in human dendritic cells. J Immunol 171:3154-62 (2003). ICC/IF, Flow Cyt, IP ; Human . PubMed: 12960343
- Matsumoto M et al. Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling. Biochem Biophys Res Commun 293:1364-9 (2002). PubMed: 12054664