• Product name
  • Description
    Rabbit polyclonal to TLR4
  • Host species
  • Specificity
    TLR4 expression levels and cleavage or degradation products can vary between different cell and tissue samples. Customers have observed this variability in WB band size and our laboratory has confirmed this variability as well observing lower molecular weight cleavage and degradation products and in some samples a lack of the full length TLR4 band. The TLR4 cleavage and degradation products and potential lack of full length TLR4 are well documented in the literature, including PMID 16885150 and 22927440. We recommend running a positive control human intestine tissue lysate. We have obtained both positive and negative feedback from researchers using this antibody with rat samples (see Abreviews). Due to the inconsistency, we have removed rat as a guaranteed species and welcome any further feedback from researchers using this antibody.
  • Tested applications
    Suitable for: IHC-P, WB, ELISA, ICC/IF, ICC, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human TLR4 aa 420-435.


    Database link: O00206

  • Positive control
    • overexpressed recombinant protein



Our Abpromise guarantee covers the use of ab13556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50.
WB 1/500.

Use primary at 1/500 dlution in 1xTBS. Incubate at 4C for 18 hours. Secondary should be incubated at 1 hour at room temperature.

ELISA Use at an assay dependent concentration. PubMed: 24952384
ICC/IF Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.


  • Function
    Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
  • Tissue specificity
    Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
  • Involvement in disease
    Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
  • Sequence similarities
    Belongs to the Toll-like receptor family.
    Contains 18 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • Domain
    The TIR domain mediates interaction with NOX4.
  • Post-translational
    N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • ARMD10 antibody
    • CD284 antibody
    • CD284 antigen antibody
    • Homolog of Drosophila toll antibody
    • hToll antibody
    • TLR 4 antibody
    • TLR4 antibody
    • TLR4_HUMAN antibody
    • TOLL antibody
    • Toll like receptor 4 antibody
    • Toll-like receptor 4 antibody
    see all


  • Anti-TLR4 antibody (ab13556) at 1/500 dilution + TLR4 transfected Baculovirus-Insect whole cell lysate at 10 µg

    Anti-Rabbit HRP conjugate at 1/2000 dilution

    Exposure time: 6 minutes
  • ab13556 staining TLR4 in human mouse spleen tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

  • ab13556 positively staining formaldehyde fixed rat primary microglia cells (1/50), used in conjunction with goat anti rabbit Alex Fluor® 488 (1/250). 
    This image is courtesy of an Abreview submitted on 3 October 2005. We do not have any further information relating to this image.

  • ab13556 diluted 1/100 detecting TLR4 transfected mouse CHO cells by flow cytometry.   The cells were prepared by treatment with collagenase and incubated with the primary antibody for 1 hour at 22°C.  An Alexa Fluor® 488 goat anti-rabbit was used as the secondary antibody.  Cells gated on live.

    See Abreview

  • ab13556 at 1/20000 dilution staining TLR4 in mouse colitis colon tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with two, 5 minutes incubation intervals in citrate buffer. An antibody amplifier™ was used for staining. A HRP-conjugated goat polyclonal to rabbit IgG was used as secondary at 1/10 dilution.

  • Flow cytometry analysis of Human monocytic Leukemia cells (THP-1). Cells were fixed with 2% Formaldehyde for 10 minutes at room temperature. Ab13556 was used at  2 µg/106 cells for 60 minutes at 37°C (green). Goat Anti- Rabbit Dylight 488 was used as a secondary antibody at 1:200 dilution for 40minutes at 37°C. Isotype control was Rabbit IgG under the same conditions (blue).


This product has been referenced in:
  • Wu XJ  et al. Penehyclidine hydrochloride inhibits TLR4 signaling and inflammation, and attenuates blunt chest trauma and hemorrhagic shock-induced acute lung injury in rats. Mol Med Rep 17:6327-6336 (2018). Read more (PubMed: 29488614) »
  • Cao W  et al. Homeobox a5 Promotes White Adipose Tissue Browning Through Inhibition of the Tenascin C/Toll-Like Receptor 4/Nuclear Factor Kappa B Inflammatory Signaling in Mice. Front Immunol 9:647 (2018). Read more (PubMed: 29651293) »

See all 56 Publications for this product

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Rhesus monkey Tissue sections (lymph node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
lymph node
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Oct 17 2017

Abcam has not validated the combination of species/application used in this Abreview.
Western blot
Cynomolgus Monkey Tissue lysate - other (macaque retina)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
300 µg
macaque retina
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 06 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Cynomolgus Monkey Tissue sections (Macaque retina)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6
Macaque retina
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 02 2016

Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 22°C
Mouse Tissue sections (frozen sections from 13.5dpc mouse embryo and plac)
frozen sections from 13.5dpc mouse embryo and plac

Mrs. Ira Rostovsky

Verified customer

Submitted Feb 16 2015

Immunohistochemistry (Frozen sections)
Marmoset (common) Tissue sections (Caudate)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Feb 21 2014

Thank you for contacting us.

I have received a response from the lab:

The antibody amplifier(TM) refers to a platform that was used. I have requestedthis productto be added to our catalog. I will let you know if this is possible. <...

Read More

We do not sell a blocking peptide for this product unfortunately.

Sorry I couldn't be more helpful here. Please contact us again if you have any further questions.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

Read More

Thank you for contacting Abcam.

I am very sorry to hear about the troubles which you have been experiencing using this product and wish to thank you for providing the details of your protocol.

Our products are covered by Abpromise gua...

Read More

Thank you very much for your interest in our TLR4 antibodies.
Based on the homology between the Sheep sequence (UniProt: B5DC89) and their immunogens, I would recommend ab47093 or ab13556, both of which are 94% homologous. Our antibody ab22048 als...

Read More

1-10 of 43 Abreviews or Q&A


Sign up