• Product name

  • Description

    Rabbit polyclonal to TLR4
  • Host species

  • Specificity

    TLR4 expression levels and cleavage or degradation products can vary between different cell and tissue samples. Customers have observed this variability in WB band size and our laboratory has confirmed this variability as well observing lower molecular weight cleavage and degradation products and in some samples a lack of the full length TLR4 band. The TLR4 cleavage and degradation products and potential lack of full length TLR4 are well documented in the literature, including PMID 16885150 and 22927440. We recommend running a positive control human intestine tissue lysate.
  • Tested applications

    Suitable for: ICC/IF, Electron Microscopy, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to TLR4 aa 39-56.


  • Positive control

    • WB: Partial recombinant mouse TLR4 (extracellular portion plus His-tag), RAW cell lysate, Daudi cell lysate



Our Abpromise guarantee covers the use of ab13867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 16959900
Electron Microscopy Use at an assay dependent concentration. PubMed: 24489676
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 75-80 kDa (predicted molecular weight: 96 kDa). Recommended lysis buffer: 10 mM Tris, pH8.0, 130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10 mM NaPi, 10 mM NaPPi (tetrasodium Pyrophosphate)(supplemented with protease inhibitor cocktail).


  • Function

    Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
  • Tissue specificity

    Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
  • Involvement in disease

    Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
  • Sequence similarities

    Belongs to the Toll-like receptor family.
    Contains 18 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • Domain

    The TIR domain mediates interaction with NOX4.
  • Post-translational

    N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • ARMD10 antibody
    • CD284 antibody
    • CD284 antigen antibody
    • Homolog of Drosophila toll antibody
    • hToll antibody
    • TLR 4 antibody
    • TLR4 antibody
    • TLR4_HUMAN antibody
    • TOLL antibody
    • Toll like receptor 4 antibody
    • Toll-like receptor 4 antibody
    see all


  • Analysis of TLR4 in mouse kidney tissue using ab13867 at 5µg/ml.

  • Western blot analysis of TLR4 using ab13867 at 2 ug/ml on A) 0.1 ug/lane partial recombinant mouse TLR4 protein and B) RAW cell lysate and C) Daudi cell lysate. Western blot analysis of TLR4 using ab13867 at 2 ug/ml on A) 0.1 ug/lane partial recombinant mouse TLR4 protein and B) RAW cell lysate (ab7187) and C) Daudi cell lysate (ab3951).


This product has been referenced in:

  • Zhang J  et al. Porphyromonas gingivalis lipopolysaccharide induces cognitive dysfunction, mediated by neuronal inflammation via activation of the TLR4 signaling pathway in C57BL/6 mice. J Neuroinflammation 15:37 (2018). WB . Read more (PubMed: 29426327) »
  • Zhang H  et al. Vitamin D reduces inflammatory response in asthmatic mice through HMGB1/TLR4/NF-?B signaling pathway. Mol Med Rep 17:2915-2920 (2018). Read more (PubMed: 29257249) »
See all 22 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Mouse Tissue sections (frozen sections from mouse embryo)
frozen sections from mouse embryo
Yes - triton-x 0.1% in TBS

Abcam user community

Verified customer

Submitted Nov 03 2014


Thank you for calling Abcam.

I have sent you a vial of ab29545, HeLa cell lysate to use as a positive control.

Please let me know if there is anything else I can help you with, or if continue to experience any issue with our antibodies.

Read More
Western blot
Mouse Tissue lysate - whole (Lungs)
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Feb 26 2009


I'm sorry to hear you are experiencing problems with ab13867. The problems you are experiencing could be due to a number of reasons, such as low levels of TLR4 in your samples (due to the transfection procedure or the extraction procedure) and therefore I would recommend to run a positive control along your samples, such as RAW cell lysate or Daudi cell lysate. I was able to find out from the laboratory the lysis buffer used to extract the receptor, it is 10 mM Tris, pH8.0, 130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10 mM NaPi, 10 mM NaPPi (tetrasodium Pyrophosphate) (supplemented with protease inhibitor cocktail). I would recommend to lyse your cells with this buffer, and leave those under agitation at 4C for several hours to give time for the receptor to be extracted efficiently. I would recommend running about 20-30ug lysate per well (reduced and boiled in loading buffer). The high background problem can be due to the blocking step, do you perform such a step at the moment? I recommend blocking for 1 hour with 5% Carnation nonfat dry milk in TBST (25 mM Tris-Cl, pH 8.0; 125 mM NaCl; 0.1% Tween 20) and incubating the antibody in 1% milk (as used when testing this antibody) or no milk. I would also try a no primary control experiment to make sure the problem is not due to the secondary antibody. You may be able to dilute the primary antibody more and incubate for longer so that the antibody binds slowly but specifically (ideally overnight at 4C). I hope the protocol information and tips I have provided will help you, as you have not mentioned certain details of your protocol (e.g blocking, antibody dilution) it is hard to recommend specific changes to make. Please do not hesitate to contact me if you require additional help,

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