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Our Abpromise guarantee covers the use of ab13867 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 16959900|
|Electron Microscopy||Use at an assay dependent concentration. PubMed: 24489676|
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 75-80 kDa (predicted molecular weight: 96 kDa). Recommended lysis buffer: 10 mM Tris, pH8.0, 130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10 mM NaPi, 10 mM NaPPi (tetrasodium Pyrophosphate)(supplemented with protease inhibitor cocktail).|
Analysis of TLR4 in mouse kidney tissue using ab13867 at 5µg/ml.
Western blot analysis of TLR4 using ab13867 at 2 ug/ml on A) 0.1 ug/lane partial recombinant mouse TLR4 protein and B) RAW cell lysate and C) Daudi cell lysate. Western blot analysis of TLR4 using ab13867 at 2 ug/ml on A) 0.1 ug/lane partial recombinant mouse TLR4 protein and B) RAW cell lysate (ab7187) and C) Daudi cell lysate (ab3951).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"