• Product name
    Anti-TLR4 antibody [HTA125]
    See all TLR4 primary antibodies
  • Description
    Mouse monoclonal [HTA125] to TLR4
  • Host species
  • Specificity
    This antibody recognises the human Toll like receptor 4 (TLR4) cell surface antigen. TLR4 has been demonstrated to act as alpha receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule.TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS. We do not guarantee WB since we receive variable results from our customers and cannot make this antibody work in our hands in WB in denaturing and reducing conditions. The immunogen is a full-length immunogen so we suspect that to get the product to work in WB, non-reducing or native WB could be a better option. THP1 monocytes or macrophage are good positive controls and HeLa/Hek293 should be suitable negative/low expression controls. Membrane preparation may be better that whole cell as expression levels may not be that high in some samples. We welcome any further feedback in WB by participating to our AbTrial program.
  • Tested applications
    Suitable for: Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Rat, Guinea pig, Human, Pig, Rhesus monkey
  • Immunogen

    Ba/F3 cell line expressing TLR4



Our Abpromise guarantee covers the use of ab8376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/25.

Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. Method sheets are available on request.




ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


IP Use at an assay dependent concentration.


  • Function
    Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
  • Tissue specificity
    Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
  • Involvement in disease
    Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
  • Sequence similarities
    Belongs to the Toll-like receptor family.
    Contains 18 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • Domain
    The TIR domain mediates interaction with NOX4.
  • Post-translational
    N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • ARMD10 antibody
    • CD284 antibody
    • CD284 antigen antibody
    • Homolog of Drosophila toll antibody
    • hToll antibody
    • TLR 4 antibody
    • TLR4 antibody
    • TLR4_HUMAN antibody
    • TOLL antibody
    • Toll like receptor 4 antibody
    • Toll-like receptor 4 antibody
    see all


  • Staining of human peripheral blood monocytes with ab8376.


This product has been referenced in:
  • Cao Z  et al. Tissue expression of Toll-like receptors 2, 3, 4 and 7 in swine in response to the Shimen strain of classical swine fever virus. Mol Med Rep 17:7122-7130 (2018). Read more (PubMed: 29568891) »
  • Peng J  et al. High-Throughput Sequencing and Co-Expression Network Analysis of lncRNAs and mRNAs in Early Brain Injury Following Experimental Subarachnoid Haemorrhage. Sci Rep 7:46577 (2017). Read more (PubMed: 28417961) »

See all 16 Publications for this product

Customer reviews and Q&As

Thank you for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appr...

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Thank you for your email.

I have checked the images and based on the images and protocol, I have following suggestions;

- Use a different HRP conjugated antibody. DAKO ChemMate EnVision Detection Kit as per DAKO website is optimized f...

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Thank you very much sending details.

I am surprised to hear that antibody failed in 2 different occasions as we haven't received any complaint for these lots so I think protocol might need further optimizations. Could you provide image of stai...

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Thank you for completing the survey on Abcam website.

I am sorry to hear that the antibody did not work with porcine sample. I have attached a questionnaire, to follow up for the complaint. Could you complete this and send it to me asap. I wil...

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Thank you for your email.

The order number I have created is 1107878; you will be receiving this antibody shortly.

TLR4 is highly expressed in porcine kidney so the antibody should have worked. Please let me know if you experience any...

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Thank you for contacting us.

I am sorry, I forgot to ask the order number of your last purchase. Could you send the purchase order number to us so that we can send the new vial at same address?

Thanks! Looking forward to hearing from ...

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Expiration date: 24-09-2012

I am very pleased to hear you would like to use ab8376 (WB) and ab11354(IHC-P) inPorcine and send us an image of you...

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Thank you for your reply. I'm afraid all the information we have is from those references and therefore we do not have any more data looking at the blocking ability of the antibody. We welcome all feedback on our antibodies via our Abreviews system ...

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I can confirm that the blocking activity of this clone has been established in a number of studies, for your convenience I have listed some useful articles below: 1: Infect Immun. 2001 Apr;69(4):2045-53. Synergistic effect of muramyldipeptide wit...

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Unfortunately I do not believe that the epitope that this antibody recognises has been fully characterised and I have been unable to locate any further information relating to the terminus recognised. I am sorry that I cannot be of additional assistanc...

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1-10 of 11 Abreviews or Q&A


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