Key features and details
- Mouse monoclonal [HTA125] to TLR4
- Suitable for: Flow Cyt, IP
- Reacts with: Rat, Guinea pig, Human, Pig, Rhesus monkey
- Isotype: IgG2a
Product nameAnti-TLR4 antibody [HTA125]
See all TLR4 primary antibodies
DescriptionMouse monoclonal [HTA125] to TLR4
SpecificityThis antibody recognises the human Toll like receptor 4 (TLR4) cell surface antigen. TLR4 has been demonstrated to act as alpha receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule.TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS. We do not guarantee WB since we receive variable results from our customers and cannot make this antibody work in our hands in WB in denaturing and reducing conditions. The immunogen is a full-length immunogen so we suspect that to get the product to work in WB, non-reducing or native WB could be a better option. THP1 monocytes or macrophage are good positive controls and HeLa/Hek293 should be suitable negative/low expression controls. Membrane preparation may be better that whole cell as expression levels may not be that high in some samples. We welcome any further feedback in WB by participating to our AbTrial program.
Tested applicationsSuitable for: Flow Cyt, IPmore details
Species reactivityReacts with: Rat, Guinea pig, Human, Pig, Rhesus monkey
Ba/F3 cell line expressing TLR4
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab8376 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/25.
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. Method sheets are available on request.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
FunctionCooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
Tissue specificityHighly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
Involvement in diseaseGenetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
Sequence similaritiesBelongs to the Toll-like receptor family.
Contains 18 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain.
DomainThe TIR domain mediates interaction with NOX4.
modificationsN-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
- Information by UniProt
- ARMD10 antibody
- CD284 antibody
- CD284 antigen antibody
ab8376 has been referenced in 26 publications.
- Kim SY et al. Isorhamnetin alleviates lipopolysaccharide-induced inflammatory responses in BV2 microglia by inactivating NF-?B, blocking the TLR4 pathway and reducing ROS generation. Int J Mol Med 43:682-692 (2019). PubMed: 30483725
- Cebatariuniene A et al. Extracellular Vesicles Suppress Basal and Lipopolysaccharide-Induced NF?B Activity in Human Periodontal Ligament Stem Cells. Stem Cells Dev N/A:N/A (2019). PubMed: 31017040
- Shi L et al. Chitosan oligosaccharide-mediated attenuation of LPS-induced inflammation in IPEC-J2 cells is related to the TLR4/NF-?B signaling pathway. Carbohydr Polym 219:269-279 (2019). PubMed: 31151525
- Cao Z et al. Tissue expression of Toll-like receptors 2, 3, 4 and 7 in swine in response to the Shimen strain of classical swine fever virus. Mol Med Rep 17:7122-7130 (2018). PubMed: 29568891
- Jin G et al. Sparstolonin B selectively suppresses toll-like receptor-2 and -4 to alleviate neuropathic pain. Mol Med Rep 17:1247-1252 (2018). PubMed: 29115627