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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-TLR4 antibody [HTA125] - Low endotoxin, Azide free (ab171260)

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Flow Cytometry - Anti-TLR4 antibody [HTA125] - Low endotoxin, Azide free (ab171260)

    Key features and details

    • Mouse monoclonal [HTA125] to TLR4 - Low endotoxin, Azide free
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Isotype: IgG2a

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    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    Protein
    Recombinant human TLR4 protein (Active) (ab233665)

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    Overview

    • Product name

      Anti-TLR4 antibody [HTA125] - Low endotoxin, Azide free
      See all TLR4 primary antibodies
    • Description

      Mouse monoclonal [HTA125] to TLR4 - Low endotoxin, Azide free
    • Host species

      Mouse
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      The details of the immunogen for this antibody are not available.

    • Positive control

      • Human peripheral blood leukocytes
    • General notes

      ab171260 has been reported for use in flow cytometric analysis. It has also been reported in blocking of LPS-induced cytokine production. For detection of peripheral monocytes, a three step staining protocol is recommended using purified anti-human TLR4 followed by biotin anti-mouse IgG and streptavidin-PE.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.2
      Constituent: PBS
    • Carrier free

      Yes
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      HTA125
    • Isotype

      IgG2a
    • Light chain type

      kappa
    • Research areas

      • Immunology
      • Innate Immunity
      • Macrophage / Inflamm.
      • Immunology
      • Immunoglobulins
      • Receptors
      • Microbiology
      • Interspecies Interaction
      • Host Virus Interaction
      • Cardiovascular
      • Atherosclerosis
      • Vascular Inflammation
      • Inflammatory mediators
      • Immunology
      • Adaptive Immunity
      • Regulatory T Cells
      • Immunology
      • Innate Immunity
      • TLR Signaling

    Associated products

    • Alternative Versions

      • PE Anti-TLR4 antibody [HTA125] (ab11227)
      • APC Anti-TLR4 antibody [HTA125], prediluted (ab155343)
      • Anti-TLR4 antibody [HTA125] - Low endotoxin, Azide free (ab30667)
      • Anti-TLR4 antibody [HTA125] (ab8376)
      • FITC Anti-TLR4 antibody [HTA125] (ab8378)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
    • Recombinant Protein

      • Recombinant human TLR4 protein (Active) (ab233665)
    • Related Products

      • Recombinant Human TLR4 protein (ab159717)

    Applications

    Our Abpromise guarantee covers the use of ab171260 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 1µg for 105-8 cells.

    A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL.  It is highly recommended that for optimal staining of TLR4, whole blood be stained using the LWB protocol rather than Ficoll-gradient prepared PBMCs. The use of a density gradient appears to reduce the staining intensity significantly.
    ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

    Target

    • Function

      Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
    • Tissue specificity

      Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
    • Involvement in disease

      Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
    • Sequence similarities

      Belongs to the Toll-like receptor family.
      Contains 18 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
      Contains 1 TIR domain.
    • Domain

      The TIR domain mediates interaction with NOX4.
    • Post-translational
      modifications

      N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession O00206 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7099 Human
      • Omim: 603030 Human
      • SwissProt: O00206 Human
      • Unigene: 174312 Human
      • Alternative names

        • ARMD10 antibody
        • CD284 antibody
        • CD284 antigen antibody
        • Homolog of Drosophila toll antibody
        • hToll antibody
        • TLR 4 antibody
        • TLR4 antibody
        • TLR4_HUMAN antibody
        • TOLL antibody
        • Toll like receptor 4 antibody
        • Toll-like receptor 4 antibody
        see all

      Images

      • Flow Cytometry - Anti-TLR4 antibody [HTA125] - Low endotoxin, Azide free (ab171260)
        Flow Cytometry - Anti-TLR4 antibody [HTA125] - Low endotoxin, Azide free (ab171260)

        Flow cytometric analysis of Human peripheral blood cells (right histogram) labeling TLR4 with ab171260  and Mouse IgG2a Isotype Control (left histogram). Cells in the monocyte population were used for analysis.

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (2)

      Publishing research using ab171260? Please let us know so that we can cite the reference in this datasheet.

      ab171260 has been referenced in 2 publications.

      • Hiraku Y  et al. Multi-walled carbon nanotube induces nitrative DNA damage in human lung epithelial cells via HMGB1-RAGE interaction and Toll-like receptor 9 activation. Part Fibre Toxicol 13:16 (2016). PubMed: 27026438
      • Yonekawa K  et al. Myeloid related proteins activate Toll-like receptor 4 in human acute coronary syndromes. Atherosclerosis 218:486-92 (2011). PubMed: 21782178

      Customer reviews and Q&As

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