Anti-TLR4 antibody [HTA125] - Low endotoxin, Azide free (ab171260)

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Flow Cytometry - Anti-TLR4 antibody [HTA125] - Low endotoxin, Azide free (ab171260)

    Key features and details

    • Mouse monoclonal [HTA125] to TLR4 - Low endotoxin, Azide free
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Isotype: IgG2a

    Overview

    • Product name

      Anti-TLR4 antibody [HTA125] - Low endotoxin, Azide free
      See all TLR4 primary antibodies

    • Description

      Mouse monoclonal [HTA125] to TLR4 - Low endotoxin, Azide free

    • Host species

      Mouse

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      The details of the immunogen for this antibody are not available.

    • Positive control

      • Human peripheral blood leukocytes

    • General notes

      ab171260 has been reported for use in flow cytometric analysis. It has also been reported in blocking of LPS-induced cytokine production. For detection of peripheral monocytes, a three step staining protocol is recommended using purified anti-human TLR4 followed by biotin anti-mouse IgG and streptavidin-PE.

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      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab171260 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 1µg for 105-8 cells.

    A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL.  It is highly recommended that for optimal staining of TLR4, whole blood be stained using the LWB protocol rather than Ficoll-gradient prepared PBMCs. The use of a density gradient appears to reduce the staining intensity significantly.
    ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

    Target

    • Function

      Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.

    • Tissue specificity

      Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.

    • Involvement in disease

      Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.

    • Sequence similarities

      Belongs to the Toll-like receptor family.
      Contains 18 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
      Contains 1 TIR domain.

    • Domain

      The TIR domain mediates interaction with NOX4.

    • Post-translational
      modifications

      N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • ARMD10 antibody
        • CD284 antibody
        • CD284 antigen antibody
        • Homolog of Drosophila toll antibody
        • hToll antibody
        • TLR 4 antibody
        • TLR4 antibody
        • TLR4_HUMAN antibody
        • TOLL antibody
        • Toll like receptor 4 antibody
        • Toll-like receptor 4 antibody
        see all

      Images

      • Flow Cytometry - Anti-TLR4 antibody [HTA125] - Low endotoxin, Azide free (ab171260)
        Flow Cytometry - Anti-TLR4 antibody [HTA125] - Low endotoxin, Azide free (ab171260)

        Flow cytometric analysis of Human peripheral blood cells (right histogram) labeling TLR4 with ab171260  and Mouse IgG2a Isotype Control (left histogram). Cells in the monocyte population were used for analysis.

      References (2)

      Publishing research using ab171260? Please let us know so that we can cite the reference in this datasheet.

      ab171260 has been referenced in 2 publications.

      • Hiraku Y  et al. Multi-walled carbon nanotube induces nitrative DNA damage in human lung epithelial cells via HMGB1-RAGE interaction and Toll-like receptor 9 activation. Part Fibre Toxicol 13:16 (2016). PubMed: 27026438
      • Yonekawa K  et al. Myeloid related proteins activate Toll-like receptor 4 in human acute coronary syndromes. Atherosclerosis 218:486-92 (2011). PubMed: 21782178

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