Anti-TLR4 antibody [HTA125] - Low endotoxin, Azide free (ab30667)
![Flow Cytometry - Anti-TLR4 antibody [HTA125] - Low endotoxin, Azide free (ab30667)](https://www.abcam.com/ps/products/30/ab30667/Images/ab30667-40604-anti-tlr4-antibody-hta125-low-endotoxin-flow-cytometry.jpg "Flow Cytometry - Anti-TLR4 antibody [HTA125] - Low endotoxin, Azide free (ab30667)")
Key features and details
- Mouse monoclonal [HTA125] to TLR4 - Low endotoxin, Azide free
- Suitable for: ICC, Flow Cyt
- Reacts with: Rat, Human
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
Anti-TLR4 antibody [HTA125] - Low endotoxin, Azide free
See all TLR4 primary antibodies -
Description
Mouse monoclonal [HTA125] to TLR4 - Low endotoxin, Azide free -
Host species
Mouse -
Specificity
ab30667 recognises the human Toll like receptor 4 (TLR4) cell surface antigen. This antibody has been demonstrated to block activation of monocytes with LPS. TLR4 expression levels and cleavage or degradation products can vary between different cell and tissue samples. Customers have observed this variability in WB band size and our laboratory has confirmed this variability as well observing lower molecular weight cleavage and degradation products and in some samples a lack of the full length TLR4 band. The TLR4 cleavage and degradation products and potential lack of full length TLR4 are well documented in the literature, including PMID 16885150 and 22927440. We recommend running a positive control human intestine tissue lysate. -
Tested applications
Suitable for: ICC, Flow Cytmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Tissue, cells or virus corresponding to TLR4. Ba/F3 cell line expressing TLR4.
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General notes
Endotoxin Level: <0.01 EU/ugThe Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
HTA125 -
Myeloma
Sp2/0 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab30667 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
|
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| Flow Cyt |
1/10 - 1/50.
Use 10µl of the suggested working dilution to label 106 cells or 100µl whole blood. |
| Notes |
|---|
|
ICC
Use at an assay dependent concentration. |
|
Flow Cyt
1/10 - 1/50. Use 10µl of the suggested working dilution to label 106 cells or 100µl whole blood. |
Target
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Function
Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific. -
Tissue specificity
Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells. -
Involvement in disease
Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 18 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain. -
Domain
The TIR domain mediates interaction with NOX4. -
Post-translational
modificationsN-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 7099 Human
- Entrez Gene: 29260 Rat
- Omim: 603030 Human
- SwissProt: O00206 Human
- SwissProt: Q9QX05 Rat
- Unigene: 174312 Human
- Unigene: 14534 Rat
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Alternative names
- ARMD10 antibody
- CD284 antibody
- CD284 antigen antibody
see all
Images
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Flow Cytometry - Anti-TLR4 antibody [HTA125] - Low endotoxin, Azide free (ab30667)ab30667 staining TLR4 in human peripheral blood monocytes by Flow Cytometry analysis.
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![Immunocytochemistry - Anti-TLR4 antibody [HTA125] - Low endotoxin, Azide free (ab30667)](https://www.abcam.com/ps/products/30/ab30667/Images/ab30667-40603-anti-tlr4-antibody-hta125-low-endotoxin-immunofluorescence.jpg)
Immunocytochemistry - Anti-TLR4 antibody [HTA125] - Low endotoxin, Azide free (ab30667)ICC/IF image of ab30667 stained rat adrenal medulla PC12 cells. The cells were 4% PFA and 100% Methanol fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30667, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
Protocols
Datasheets and documents
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Datasheet download
References (46)
ab30667 has been referenced in 46 publications.
- Li Q et al. Qiang-Gan formula extract improves non-alcoholic steatohepatitis via regulating bile acid metabolism and gut microbiota in mice. J Ethnopharmacol 258:112896 (2020). PubMed: 32325178
- Li Q et al. Marrow-derived mesenchymal stem cells regulate the inflammatory response and repair alveolar type II epithelial cells in acute lung injury of rats. J Int Med Res 48:300060520909027 (2020). PubMed: 32314638
- Xu C et al. Thrombospondin 2/Toll-Like Receptor 4 Axis Contributes to HIF-1a-Derived Glycolysis in Colorectal Cancer. Front Oncol 10:557730 (2020). PubMed: 33244454
- Chu KA et al. Comparison of reversal of rat pulmonary fibrosis of nintedanib, pirfenidone, and human umbilical mesenchymal stem cells from Wharton's jelly. Stem Cell Res Ther 11:513 (2020). PubMed: 33256831
- Giridharan VV et al. Maternal deprivation increases microglial activation and neuroinflammatory markers in the prefrontal cortex and hippocampus of infant rats. J Psychiatr Res 115:13-20 (2019). PubMed: 31082652
