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I want to use ab30667, TLR4 antibody [HTA125] - Azide free in Blocking assay. Could you show me the recommended protocol and dilution for that?
Asked on Jun 11 2010
Thank you for your question about ab30667. Generally, functional assays can cover a number of different experiments. However, this antibody has been used in blocking studies. As these will each be slightly different, we cannot provide a protocol, or give a recommended protocol. Instead we would recommend checking the literature for further information on using the same clone antibody (clone HTA125) in functional assays: 1) Shimazu, R. et al. (1999) MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll like receptor 4. J. Exp. Med. 189: 1777-1182.[http://www.ncbi.nlm.nih.gov/pubmed/10359581] 2) Yang, S. et al. (2001) Synergistic effect of muramyldipeptide with lipopolysaccharide or lipoteichoic acid to induce inflammatory cytokines in human monocytic cells in culture. Infect. Immun. 69: 2045-2053.[http://www.ncbi.nlm.nih.gov/pubmed/11254557] 3) Sugawara, S. et al. (2000). Proteolysis of human monocyte CD14 by cysteine proteinases (gingipains) from Porphyromonas gingivalis leading to lipopolysaccharide hyporesponsiveness. J. Immunol. 165: 411-418.[http://www.ncbi.nlm.nih.gov/pubmed/10861079] 4) Wang, J.E. et al. (2001) Involvement of CD14 and toll-like receptors in activation of human monocytes by Aspergillus fumigatus hyphae.[http://www.ncbi.nlm.nih.gov/pubmed/11254600] Infect Immun. 69: 2402-2406.[http://www.ncbi.nlm.nih.gov/pubmed/11254600] 5) Termeer, C. et al. (2002) Oligosaccharides of Hyaluronan activate dendritic cells via toll-like receptor 4. J Exp Med. 195: 99-111.[http://www.ncbi.nlm.nih.gov/pubmed/11781369] I hope that this information is helpful. Please do not hesitate to contact us if you have further questions.
Answered on Jun 11 2010