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Is this TLR4antibody expected to react with bovine protein?
Asked on Sep 11 2012
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The immunogen for ab30667, clone HTA125, was human TLR4-transfected mouse cell line Ba/F3, according to the paper describing development of the clone at the following URL, Shimazu et al, 1999:
Alignment of the amino acid sequences for human and bovine TLR4 (http://www.uniprot.org/uniprot/O00206 and http://www.uniprot.org/uniprot/Q9GL65 UniProt entries)
returns 76% identity. As I mentioned, if the epitope the antibody recognizes is conserved across both species, then we expect the antibody will cross-react, but there are many mismatches which the antibody would be unable to bind to on the bovine protein. Unfortunately, the epitope has not been mapped to a region with the the full-length sequence, which would allow a more precise assessment of potential for cross-reactivity.
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Answered on Sep 11 2012