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Inquiry: I would like to know if the TLR4 antibody [HTA125] (ab30667) is directed at the N or C terminus. Also, what are the benefits of having an antibody specific to the TLR4 N terminus vs. the TLR4 C terminus?
Asked on Oct 25 2012
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Immunogen is human TLR4-transfected mouse cell line Ba/F3, http://jem.rupress.org/content/189/11/1777.long
No epitope mapping has been performed, but based on application info for this antibody, it should be recognizing an extracellular epitope as no cell permeabilization is needed for flow cytometry. The extracellular domain is towards the N terminal and is more varied than the cytoplasmic domain at the C terminal; there are three isofrms of TLR4 whichdiffer in the first 200 amino acids and have varied molecular weights.
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Answered on Oct 25 2012