• Product name

    Anti-TLR4 antibody [HTA125] (Phycoerythrin)
    See all TLR4 primary antibodies
  • Description

    Mouse monoclonal [HTA125] to TLR4 (Phycoerythrin)
  • Host species

  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human, Rhesus monkey
  • Immunogen

    Ba/F3 cell line expressing human TLR4.

  • Positive control

    • Human monocytes and macrophages/whole blood.
  • General notes

    This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

    TLR4 expression on U937 cells can be upregulated following stimulation with e.g. LPS or IFN gamma.



Our Abpromise guarantee covers the use of ab11227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/1.

Use 10 µl of undiluted antibody to label 106 cells or 100 µl whole blood.

ab91363 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


  • Function

    Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
  • Tissue specificity

    Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
  • Involvement in disease

    Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
  • Sequence similarities

    Belongs to the Toll-like receptor family.
    Contains 18 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • Domain

    The TIR domain mediates interaction with NOX4.
  • Post-translational

    N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • ARMD10 antibody
    • CD284 antibody
    • CD284 antigen antibody
    • Homolog of Drosophila toll antibody
    • hToll antibody
    • TLR 4 antibody
    • TLR4 antibody
    • TLR4_HUMAN antibody
    • TOLL antibody
    • Toll like receptor 4 antibody
    • Toll-like receptor 4 antibody
    see all


  • Staining of U937 cells with ab11227.
  • Ab11227 staining on human peripheral blood monocytes.


ab11227 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


Thank you for your email. We are unable to measure the concentration of our PE conjugates, so I cannot give you precise details. However, the concentration of the antibody will be approximately 0.1mg/ml. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Thank you for your enquiry. Expression of TLR4 on normal resting monocytes is low and for this reason ab11227 is routinely QC'd on the U937 cell line which demonstrates a reasonable level of expression. I can attach an image here so I will send you a separate email with some histograms. However, I am pleased to say that I was able to obtain a histogram showing our current batch of ab11227 tested on monocytes. Expression of TLR4 is low, overlapping the isotype control, but still distinct. I believe that TLR4 expression on this cell type can be upregulated following stimulation with e.g. LPS or IFN gamma. You could refer to the literature for further information but the following paper will provide a good starting point. Bosisio, D. et al. (2002). Stimulation of toll-like receptor 4 expression in human mononuclear phagocytes by interferon-gamma: a molecular basis for priming and synergism with bacterial lipopolysaccharide. Blood. 99(9):3427-3431. Link to full text: http://www.bloodjournal.org/cgi/reprint/99/9/3427 I will send you a separate email with the histograms. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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