Key features and details
- Rabbit polyclonal to TLR4/MD2 Complex
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TLR4/MD2 Complex antibody
See all TLR4/MD2 Complex primary antibodies
DescriptionRabbit polyclonal to TLR4/MD2 Complex
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Cow, Macaque monkey
- Human prostate tissue; Human intestine tissue lysate.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab218987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 95 kDa.|
FunctionCooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
Tissue specificityHighly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
Involvement in diseaseGenetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
Sequence similaritiesBelongs to the Toll-like receptor family.
Contains 18 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain.
DomainThe TIR domain mediates interaction with NOX4.
modificationsN-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
- Information by UniProt
- ARMD10 antibody
- CD284 antibody
- CD284 antigen antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue, labeling TLR4/MD2 Complex with ab218987 at 5 μg/ml.
All lanes : Anti-TLR4/MD2 Complex antibody (ab218987) at 0.1 µg/ml
Lane 1 : Human intestine lysate
Lane 2 : Human intestine lysate with Immunizing peptide
All lanes : Goat anti-Rabbit Ig HRP
Predicted band size: 95 kDa
ab218987 has not yet been referenced specifically in any publications.