Key features and details
- Rat monoclonal [MTS510] to TLR4/MD2 Complex (Biotin)
- Suitable for: IP, Flow Cyt, IHC-Fr, Functional Studies
- Reacts with: Mouse
- Conjugation: Biotin
- Isotype: IgG2a
Product nameAnti-TLR4/MD2 Complex antibody [MTS510] (Biotin)
See all TLR4/MD2 Complex primary antibodies
DescriptionRat monoclonal [MTS510] to TLR4/MD2 Complex (Biotin)
SpecificityOnly detects murine TLR-4 when complexed to MD-2. This antibody has been found to coprecipitate MD-2 (30kDa) with TLR4 (100kDa).
Tested applicationsSuitable for: IP, Flow Cyt, IHC-Fr, Functional Studiesmore details
Species reactivityReacts with: Mouse
Mouse Ba/F3 cells expressing mouse TLR4/MD-2
Ab9105 is a TLR4 function blocking antibody that is useful for studies on the role of TLR4 as a receptor fpr LPS induced cytokine production by TLR4 bearing cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityProtein G purified
Purification notes0.2 µm filtered
Primary antibody notesAb9105 is a TLR4 function blocking antibody that is useful for studies on the role of TLR4 as a receptor fpr LPS induced cytokine production by TLR4 bearing cells.
Our Abpromise guarantee covers the use of ab9105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent concentration.|
FunctionCooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
Tissue specificityHighly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
Involvement in diseaseGenetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
Sequence similaritiesBelongs to the Toll-like receptor family.
Contains 18 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain.
DomainThe TIR domain mediates interaction with NOX4.
modificationsN-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
- Information by UniProt
- ARMD10 antibody
- CD284 antibody
- CD284 antigen antibody
ab9105 has been referenced in 2 publications.
- Chakraborty D et al. Alarmin S100A8 Activates Alveolar Epithelial Cells in the Context of Acute Lung Injury in a TLR4-Dependent Manner. Front Immunol 8:1493 (2017). PubMed: 29180999
- Daubeuf B et al. TLR4/MD-2 monoclonal antibody therapy affords protection in experimental models of septic shock. J Immunol 179:6107-14 (2007). Flow Cyt ; Mouse . PubMed: 17947685