Key features and details
- Rat monoclonal [MTS510] to TLR4/MD2 Complex (PE)
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2a
Product nameAnti-TLR4/MD2 Complex antibody [MTS510] (PE)
See all TLR4/MD2 Complex primary antibodies
DescriptionRat monoclonal [MTS510] to TLR4/MD2 Complex (PE)
ConjugationPE. Ex: 488nm, Em: 575nm
SpecificityThe MTS510 monoclonal antibody co-immunoprecipitates MD-2 (~30 kDa) and TLR4 (~100 kDa), and preferentially reacts with TLR4 that is associated with MD-2.
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse
Toll-like receptor 4 (TLR4)/MD-2 complex of mouse origin
- 3-day thioglycolate-induced BALB/c macrophages; mouse peritoneal exudate cells
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, PBS
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab95563 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.5µg for 105-8 cells.
Staining the cell sample in a final volume of 100 µL is recommended.
ab134674 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionCooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
Tissue specificityHighly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
Involvement in diseaseGenetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
Sequence similaritiesBelongs to the Toll-like receptor family.
Contains 18 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain.
DomainThe TIR domain mediates interaction with NOX4.
modificationsN-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
- Information by UniProt
- ARMD10 antibody
- CD284 antibody
- CD284 antigen antibody
ab95563 has been referenced in 4 publications.
- Chabot S et al. TLRs regulate the gatekeeping functions of the intestinal follicle-associated epithelium. J Immunol 176:4275-83 (2006). PubMed: 16547265
- Mortaz E et al. Acetylsalicylic acid-induced release of HSP70 from mast cells results in cell activation through TLR pathway. Exp Hematol 34:8-18 (2006). PubMed: 16413386
- Qi HY & Shelhamer JH Toll-like receptor 4 signaling regulates cytosolic phospholipase A2 activation and lipid generation in lipopolysaccharide-stimulated macrophages. J Biol Chem 280:38969-75 (2005). PubMed: 16176925
- Ortega-Cava CF et al. Strategic compartmentalization of Toll-like receptor 4 in the mouse gut. J Immunol 170:3977-85 (2003). PubMed: 12682225