Product nameAnti-TLR5 antibody [19D759.2]
See all TLR5 primary antibodies
DescriptionMouse monoclonal [19D759.2] to TLR5
SpecificityThis antibody recognizes an intracellular epitope in the cytoplasmic domain of TLR5.
Tested applicationsSuitable for: WB, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, IP, ICCmore details
Species reactivityReacts with: Mouse, Human
- Ramos and Raw cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
Purification notesPurified from ascites.
Light chain typekappa
Our Abpromise guarantee covers the use of ab13876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 103 kDa.|
|Flow Cyt||Use 2µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Antigen retrieval with citrate buffer pH 6.|
|IHC-Fr||Use a concentration of 10 µg/ml. (see Miller et al).|
|IP||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
FunctionParticipates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
Tissue specificityHighly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis.
Involvement in diseaseSystemic lupus erythematosus 1
Sequence similaritiesBelongs to the Toll-like receptor family.
Contains 22 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain.
modificationsPhosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines.
Phosphorylated at Tyr-798 upon flagellin binding; required for signaling.
- Information by UniProt
- FLJ10052 antibody
- MGC126430 antibody
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Intracellular FACS analysis of TLR5 in 106 Ramos cells using (A) 2
µg of isotype control and (B) 2 µg of ab13876
All lanes : Anti-TLR5 antibody [19D759.2] (ab13876) at 2 µg/ml
Lane 1 : Ramos cell lysate
Lane 2 : Raw cell lysate
Predicted band size: 103 kDa
Western blot analysis of TLR5 in human Ramos cell lysate (A) and mouse Raw cell,lysate (B) using ab13876 at 2
ab13876 staining TLR5 in Human stomach tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 1 hour at 23°C; antigen retrieval was by heat mediation in citrate buffer (10mM, pH 6). Samples were incubated with primary antibody (10 µg/ml) for 1 hour at 23°C. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.
ab13876 staining TLR5 in Human stomach tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 5% serum for 1 hour at 23°C. Samples were incubated with primary antibody (10 µg/ml in blocking buffer) for 1 hour at 23°C. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.
This product has been referenced in:
- Yamada K et al. Upregulation of Toll-like receptor 5 expression in the conjunctival epithelium of various human ocular surface diseases. Br J Ophthalmol N/A:N/A (2014). Human . Read more (PubMed: 24820048) »
- Dill-McFarland KA et al. Hibernation alters the diversity and composition of mucosa-associated bacteria while enhancing antimicrobial defence in the gut of 13-lined ground squirrels. Mol Ecol 23:4658-69 (2014). Squirrel . Read more (PubMed: 25130694) »