Key features and details
- Mouse monoclonal [19D759.2] to TLR5
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG2a
Product nameAnti-TLR5 antibody [19D759.2]
See all TLR5 primary antibodies
DescriptionMouse monoclonal [19D759.2] to TLR5
SpecificityThis antibody recognizes an intracellular epitope in the cytoplasmic domain of TLR5.
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Mouse, Human
- Ramos and Raw cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab13876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 103 kDa.|
|Flow Cyt||Use 2µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionParticipates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
Tissue specificityHighly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis.
Involvement in diseaseSystemic lupus erythematosus 1
Sequence similaritiesBelongs to the Toll-like receptor family.
Contains 22 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain.
modificationsPhosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines.
Phosphorylated at Tyr-798 upon flagellin binding; required for signaling.
- Information by UniProt
- FLJ10052 antibody
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Intracellular FACS analysis of TLR5 in 106 Ramos cells using (A) 2
µg of isotype control and (B) 2 µg of ab13876
All lanes : Anti-TLR5 antibody [19D759.2] (ab13876) at 2 µg/ml
Lane 1 : Ramos cell lysate
Lane 2 : Raw cell lysate
Predicted band size: 103 kDa
Western blot analysis of TLR5 in human Ramos cell lysate (A) and mouse Raw cell,lysate (B) using ab13876 at 2
ab13876 has been referenced in 8 publications.
- Sun M et al. The prognostic value of toll-like receptor5 and programmed cell death-ligand1 in patients with peripheral T-cell non-Hodgkin lymphoma. Leuk Lymphoma N/A:1-12 (2019). PubMed: 31017033
- Oh SH et al. Enhancement of antigen-specific humoral immune responses and protein solubility through conjugation of bacterial flagellin, Vibrio vulnificus FlaB, to the N-terminus of porcine epidemic diarrhea virus surface protein antigen S0. J Vet Sci 20:e70 (2019). PubMed: 31775197
- Yamada K et al. Upregulation of Toll-like receptor 5 expression in the conjunctival epithelium of various human ocular surface diseases. Br J Ophthalmol N/A:N/A (2014). Human . PubMed: 24820048
- Dill-McFarland KA et al. Hibernation alters the diversity and composition of mucosa-associated bacteria while enhancing antimicrobial defence in the gut of 13-lined ground squirrels. Mol Ecol 23:4658-69 (2014). Squirrel . PubMed: 25130694
- Kojima K et al. Human conjunctival epithelial cells express functional Toll-like receptor 5. Br J Ophthalmol 92:411-6 (2008). PubMed: 18211936
- Hozono Y et al. Human corneal epithelial cells respond to ocular-pathogenic, but not to nonpathogenic-flagellin. Biochem Biophys Res Commun 347:238-47 (2006). Flow Cyt ; Human . PubMed: 16824488
- François S et al. Inhibition of neutrophil apoptosis by TLR agonists in whole blood: involvement of the phosphoinositide 3-kinase/Akt and NF-kappaB signaling pathways, leading to increased levels of Mcl-1, A1, and phosphorylated Bad. J Immunol 174:3633-42 (2005). PubMed: 15749901
- Miller LS et al. TGF-alpha regulates TLR expression and function on epidermal keratinocytes. J Immunol 174:6137-43 (2005). PubMed: 15879109