Product nameAnti-TLR5 antibody
See all TLR5 primary antibodies
DescriptionRabbit polyclonal to TLR5
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Sheep, Rabbit, Goat, Cow, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide within Mouse TLR5 aa 400-500 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q9JLF7
- This antibody gave a positive signal in both Mouse and Human Liver tissue lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab156364 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250. Detects a band of approximately 100 kDa (predicted molecular weight: 97 kDa).|
FunctionParticipates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
Tissue specificityHighly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis.
Involvement in diseaseSystemic lupus erythematosus 1
Sequence similaritiesBelongs to the Toll-like receptor family.
Contains 22 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain.
modificationsPhosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines.
Phosphorylated at Tyr-798 upon flagellin binding; required for signaling.
- Information by UniProt
- FLJ10052 antibody
- MGC126430 antibody
- MGC126431 antibody
All lanes : Anti-TLR5 antibody (ab156364) at 1/250 dilution
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa (possible non-specific binding), 50 kDa (possible non-specific binding)
Exposure time: 8 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab156364 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab156364 has not yet been referenced specifically in any publications.