Anti-TLR5 antibody (ab62460)
Key features and details
- Rabbit polyclonal to TLR5
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-TLR5 antibody
See all TLR5 primary antibodies -
Description
Rabbit polyclonal to TLR5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat -
Immunogen
A 16 amino acid synthetic peptide near the center of human TLR5
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Positive control
- Rat brain lysate, brain tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
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IHC-P | (3) |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa). |
IHC-P
Use a concentration of 10 µg/ml. |
Target
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Function
Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
Tissue specificity
Highly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis. -
Involvement in disease
Systemic lupus erythematosus 1 -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 22 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain. -
Post-translational
modificationsPhosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines.
Phosphorylated at Tyr-798 upon flagellin binding; required for signaling. -
Cellular localization
Membrane. - Information by UniProt
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Database links
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Alternative names
- FLJ10052 antibody
- MGC126430 antibody
- MGC126431 antibody
see all
Images
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Immunohistochemistry of TLR5 on paraffin embedded rat brain tissue section with ab62460 at 10 µg/ml.
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Lane 1 : Anti-TLR5 antibody (ab62460) at 0.5 µg/ml
Lane 2 : Anti-TLR5 antibody (ab62460) at 1 µg/ml
Lane 3 : Anti-TLR5 antibody (ab62460) at 2 µg/ml
All lanes : Rat brain cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 97 kDa
Observed band size: 97 kDa -
ab62460 staining TLR5 in human pancreatic tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then incubated with the primary antibody at a 1/50 dilution for 14 hours at 4°C. An undiluted HRP-conjugated goat polyclonal was used as secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab62460 has been referenced in 11 publications.
- Tian X et al. Expression of TLR2 and TLR5 in distal ileum of mice with obstructive jaundice and their role in intestinal mucosal injury. Arch Med Sci 18:237-250 (2022). PubMed: 35154543
- Rao K et al. Shenling Baizhu Powder Alleviates TNBS-Induced Colitis in Rats by Improving Intestinal Epithelial Permeability and Inhibiting Inflammation Through the TLR5/MyD88/NF-κB Pathway. Front Pharmacol 13:883918 (2022). PubMed: 35571126
- Min HK et al. Relationship between toll-like receptor expression in the distal facial nerve and facial nerve recovery after injury. Int J Immunopathol Pharmacol 36:3946320221090007 (2022). PubMed: 35585682
- Zhang J et al. Expression and function of Toll-like receptors in peripheral blood mononuclear cells in patients with ankylosing spondylitis. Mol Med Rep 20:3565-3572 (2019). PubMed: 31485664
- Moroi H et al. The expression of Toll-like receptor 5 in preterm histologic chorioamnionitis. J Clin Biochem Nutr 62:63-67 (2018). PubMed: 29371755