Key features and details
- Rabbit polyclonal to TLR5
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-TLR5 antibody
See all TLR5 primary antibodies
DescriptionRabbit polyclonal to TLR5
SpecificityThis antibody reacts with TLR5
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
A 16 amino acid synthetic peptide near the center of human TLR5
- Rat brain lysate, brain tissue
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab62460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).|
|IHC-P||Use a concentration of 10 µg/ml.|
FunctionParticipates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
Tissue specificityHighly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis.
Involvement in diseaseSystemic lupus erythematosus 1
Sequence similaritiesBelongs to the Toll-like receptor family.
Contains 22 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain.
modificationsPhosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines.
Phosphorylated at Tyr-798 upon flagellin binding; required for signaling.
- Information by UniProt
- FLJ10052 antibody
- MGC126430 antibody
- MGC126431 antibody
Lane 1 : Anti-TLR5 antibody (ab62460) at 0.5 µg/ml
Lane 2 : Anti-TLR5 antibody (ab62460) at 1 µg/ml
Lane 3 : Anti-TLR5 antibody (ab62460) at 2 µg/ml
All lanes : Rat brain cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 97 kDa
Observed band size: 97 kDa
Immunohistochemistry of TLR5 on paraffin embedded rat brain tissue section with ab62460 at 10 µg/ml.
ab62460 staining TLR5 in human pancreatic tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then incubated with the primary antibody at a 1/50 dilution for 14 hours at 4°C. An undiluted HRP-conjugated goat polyclonal was used as secondary antibody.
ab62460 has been referenced in 8 publications.
- Zhang J et al. Expression and function of Toll-like receptors in peripheral blood mononuclear cells in patients with ankylosing spondylitis. Mol Med Rep 20:3565-3572 (2019). PubMed: 31485664
- Moroi H et al. The expression of Toll-like receptor 5 in preterm histologic chorioamnionitis. J Clin Biochem Nutr 62:63-67 (2018). PubMed: 29371755
- Jiang C et al. The Expression of Toll-like receptors in eutopic and ectopic endometrium and its implication in the inflammatory pathogenesis of adenomyosis. Sci Rep 7:7365 (2017). PubMed: 28779087
- Gibson RJ et al. Chemotherapy-induced gut toxicity and pain: involvement of TLRs. Support Care Cancer 24:2251-2258 (2016). PubMed: 26581898
- Feng Y et al. Role of Toll-like receptors in diabetic renal lesions in a miniature pig model. Sci Adv 1:e1400183 (2015). PubMed: 26601192
- Zheng B et al. Transcriptional modulation of pattern recognition receptors in acute colitis in mice. Biochim Biophys Acta 1832:2162-2172 (2013). IHC-P ; Mouse . PubMed: 23851050
- Weile C et al. Glucose activation of islets of Langerhans up-regulates Toll-like receptor 5: possible mechanism of protection. Clin Exp Immunol 166:251-7 (2011). PubMed: 21985371
- Rydberg C et al. Toll-like receptor agonists induce inflammation and cell death in a model of head and neck squamous cell carcinomas. Immunology 128:e600-11 (2009). IHC-P ; Human . PubMed: 19740321