Overview

  • Product name

    Anti-TLR5 antibody - N-terminal
    See all TLR5 primary antibodies
  • Description

    Rabbit polyclonal to TLR5 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human TLR5 (N terminal). The exact sequence is proprietary.
    Database link: O60602

  • Positive control

    • WB: THP-1 and NCI-H929 whole cell lysates. IHC-P: Human breast cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab228493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 97 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificity

    Highly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis.
  • Involvement in disease

    Systemic lupus erythematosus 1
  • Sequence similarities

    Belongs to the Toll-like receptor family.
    Contains 22 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • Post-translational
    modifications

    Phosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines.
    Phosphorylated at Tyr-798 upon flagellin binding; required for signaling.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ10052 antibody
    • MGC126430 antibody
    • MGC126431 antibody
    • SLEB1 antibody
    • TIL 3 antibody
    • TIL3 antibody
    • TLR 5 antibody
    • Tlr5 antibody
    • TLR5_HUMAN antibody
    • Toll like receptor 5 antibody
    • Toll like receptor 5 precursor antibody
    • Toll-like receptor 5 antibody
    • Toll/interleukin 1 receptor like protein 3 antibody
    • Toll/interleukin-1 receptor-like protein 3 antibody
    see all

Images

  • All lanes : Anti-TLR5 antibody - N-terminal (ab228493) at 1/1000 dilution

    Lane 1 : THP-1 (human monocytic leukemia cell line) whole cell lysate
    Lane 2 : NCI-H929 (human lymphoblast cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 97 kDa



    7.5% SDS-PAGE

  • Paraffin-embedded human breast cancer tissue stained for TLR5 with ab228493 at 1:250 dilution in immunohistochemical analysis.

References

ab228493 has not yet been referenced specifically in any publications.

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