Product nameAnti-TLR5 antibody - N-terminal
See all TLR5 primary antibodies
DescriptionRabbit polyclonal to TLR5 - N-terminal
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey
Recombinant fragment within Human TLR5 (N terminal). The exact sequence is proprietary.
Database link: O60602
- WB: THP-1 and NCI-H929 whole cell lysates. IHC-P: Human breast cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 97 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionParticipates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
Tissue specificityHighly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis.
Involvement in diseaseSystemic lupus erythematosus 1
Sequence similaritiesBelongs to the Toll-like receptor family.
Contains 22 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain.
modificationsPhosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines.
Phosphorylated at Tyr-798 upon flagellin binding; required for signaling.
- Information by UniProt
- FLJ10052 antibody
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All lanes : Anti-TLR5 antibody - N-terminal (ab228493) at 1/1000 dilution
Lane 1 : THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 2 : NCI-H929 (human lymphoblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 97 kDa
Paraffin-embedded human breast cancer tissue stained for TLR5 with ab228493 at 1:250 dilution in immunohistochemical analysis.
ab228493 has not yet been referenced specifically in any publications.