Overview

  • Product name

  • Description

    Rabbit polyclonal to TLR6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Fusion protein within Human TLR6 (internal sequence). The exact sequence is proprietary.
    Database link: Q9Y2C9

  • Positive control

    • Human thyroid cancer and breast cancer tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab197807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200.

Target

  • Function

    Participates in the innate immune response to Gram-positive bacteria and fungi. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR2.
  • Tissue specificity

    Detected in monocytes, CD11c+ immature dendritic cells, plasmacytoid pre-dendritic cells and dermal microvessel endothelial cells.
  • Sequence similarities

    Belongs to the Toll-like receptor family.
    Contains 12 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • Cellular localization

    Cell membrane. Cytoplasmic vesicle > phagosome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD286 antibody
    • CD286 antigen antibody
    • TLR 6 antibody
    • Tlr6 antibody
    • TLR6_HUMAN antibody
    • Toll like receptor 6 precursor antibody
    • Toll-like receptor 6 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling TLR6 using ab197807 at a 1/25 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling TLR6 using ab197807 at a 1/25 dilution.

References

ab197807 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Western blot
Sample
Cynomolgus monkey Tissue lysate - whole (macaque retina)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
300 µg
Specification
macaque retina
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 22 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (muller cell line MIO-M1)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
200 µg
Specification
muller cell line MIO-M1
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 05 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (muller cell line MIO-M1)
Permeabilization
Yes - 0.1% TX-100
Specification
muller cell line MIO-M1
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 27 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cynomolgus Monkey Tissue sections (Macaque retina tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH 6
Permeabilization
No
Specification
Macaque retina tissue
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 10 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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