Product nameAnti-TLR6 antibody
See all TLR6 primary antibodies
DescriptionRabbit polyclonal to TLR6
SpecificityTLR6 antibody is specific to TLR6. It does not recognize TLR1.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to 15 amino acids near the amino terminus of the human TLR6 protein.
- Jurkat whole cell lysate (ab7899).
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was purified by affinity chromatography.
Our Abpromise guarantee covers the use of ab37072 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 92 kDa).|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionParticipates in the innate immune response to Gram-positive bacteria and fungi. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR2.
Tissue specificityDetected in monocytes, CD11c+ immature dendritic cells, plasmacytoid pre-dendritic cells and dermal microvessel endothelial cells.
Sequence similaritiesBelongs to the Toll-like receptor family.
Contains 12 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain.
Cellular localizationCell membrane. Cytoplasmic vesicle > phagosome membrane.
- Information by UniProt
- CD286 antibody
- CD286 antigen antibody
- TLR 6 antibody
Western Blot of Daudi cell lysate labeling TLR6 with Anti-TLR6 antibody (ab37072) at 1µg/ml in (1) the absence and (2) the presence of blocking peptide..
Lane 1 : Anti-TLR6 antibody (ab37072) at 0.5 µg/ml
Lane 2 : Anti-TLR6 antibody (ab37072) at 1 µg/ml
Lane 3 : Anti-TLR6 antibody (ab37072) at 2 µg/ml
All lanes : Jurkat cell lysate
Lysates/proteins at 15 µg per lane.
All lanes : goat anti-rabbit HRP at 1/10000 dilution
Predicted band size: 92 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
ab37072 at 10 µg/ml staining TLR6 in Jurkat cells by ICC/IF
This product has been referenced in:
- Galoian K et al. Toll like receptors TLR1/2, TLR6 and MUC5B as binding interaction partners with cytostatic proline rich polypeptide 1 in human chondrosarcoma. Int J Oncol 52:139-154 (2018). Read more (PubMed: 29138803) »
- Moretti IF et al. Plasmatic membrane toll-like receptor expressions in human astrocytomas. PLoS One 13:e0199211 (2018). Read more (PubMed: 29912993) »