Key features and details
- Rabbit polyclonal to TLR6
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-TLR6 antibody
See all TLR6 primary antibodies
DescriptionRabbit polyclonal to TLR6
SpecificityTLR6 antibody is specific to TLR6. It does not recognize TLR1.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to 15 amino acids near the amino terminus of the human TLR6 protein.
- Jurkat whole cell lysate (ab7899).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was purified by affinity chromatography.
Our Abpromise guarantee covers the use of ab37072 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 92 kDa).|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionParticipates in the innate immune response to Gram-positive bacteria and fungi. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR2.
Tissue specificityDetected in monocytes, CD11c+ immature dendritic cells, plasmacytoid pre-dendritic cells and dermal microvessel endothelial cells.
Sequence similaritiesBelongs to the Toll-like receptor family.
Contains 12 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain.
Cellular localizationCell membrane. Cytoplasmic vesicle > phagosome membrane.
- Information by UniProt
- CD286 antibody
- CD286 antigen antibody
- TLR 6 antibody
Western Blot of Daudi cell lysate labeling TLR6 with Anti-TLR6 antibody (ab37072) at 1µg/ml in (1) the absence and (2) the presence of blocking peptide..
Lane 1 : Anti-TLR6 antibody (ab37072) at 0.5 µg/ml
Lane 2 : Anti-TLR6 antibody (ab37072) at 1 µg/ml
Lane 3 : Anti-TLR6 antibody (ab37072) at 2 µg/ml
All lanes : Jurkat cell lysate
Lysates/proteins at 15 µg per lane.
All lanes : goat anti-rabbit HRP at 1/10000 dilution
Predicted band size: 92 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
ab37072 at 10 µg/ml staining TLR6 in Jurkat cells by ICC/IF
ab37072 has been referenced in 5 publications.
- Galoian K et al. Toll like receptors TLR1/2, TLR6 and MUC5B as binding interaction partners with cytostatic proline rich polypeptide 1 in human chondrosarcoma. Int J Oncol 52:139-154 (2018). PubMed: 29138803
- Moretti IF et al. Plasmatic membrane toll-like receptor expressions in human astrocytomas. PLoS One 13:e0199211 (2018). PubMed: 29912993
- Salyer ACD & David SA Transcriptomal signatures of vaccine adjuvants and accessory immunostimulation of sentinel cells by toll-like receptor 2/6 agonists. Hum Vaccin Immunother 14:1686-1696 (2018). PubMed: 29852079
- Ma L et al. Developmental expression of Toll-like receptors in the guinea pig lung. Mol Med Rep 15:1243-1251 (2017). PubMed: 28098883
- Choteau L et al. Role of TLR1, TLR2 and TLR6 in the modulation of intestinal inflammation and Candida albicans elimination. Gut Pathog 9:9 (2017). PubMed: 28289440