Overview

  • Product name
  • Description
    Rabbit polyclonal to TLR6
  • Host species
    Rabbit
  • Specificity
    TLR6 antibody is specific to TLR6. It does not recognize TLR1.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to 15 amino acids near the amino terminus of the human TLR6 protein.

  • Positive control
    • Jurkat whole cell lysate (ab7899).

Properties

Applications

Our Abpromise guarantee covers the use of ab37072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 92 kDa).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Participates in the innate immune response to Gram-positive bacteria and fungi. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR2.
  • Tissue specificity
    Detected in monocytes, CD11c+ immature dendritic cells, plasmacytoid pre-dendritic cells and dermal microvessel endothelial cells.
  • Sequence similarities
    Belongs to the Toll-like receptor family.
    Contains 12 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • Cellular localization
    Cell membrane. Cytoplasmic vesicle > phagosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD286 antibody
    • CD286 antigen antibody
    • TLR 6 antibody
    • Tlr6 antibody
    • TLR6_HUMAN antibody
    • Toll like receptor 6 precursor antibody
    • Toll-like receptor 6 antibody
    see all

Images

  • Western Blot of Daudi cell lysate labeling TLR6 with Anti-TLR6 antibody (ab37072) at 1µg/ml in (1) the absence and (2) the presence of blocking peptide..
  • Lane 1 : Anti-TLR6 antibody (ab37072) at 0.5 µg/ml
    Lane 2 : Anti-TLR6 antibody (ab37072) at 1 µg/ml
    Lane 3 : Anti-TLR6 antibody (ab37072) at 2 µg/ml

    All lanes : Jurkat cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/10000 dilution

    Predicted band size: 92 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 55 kDa, 80 kDa. We are unsure as to the identity of these extra bands.

  • ab37072 at 10 µg/ml staining TLR6 in Jurkat cells by ICC/IF

References

This product has been referenced in:
  • Galoian K  et al. Toll like receptors TLR1/2, TLR6 and MUC5B as binding interaction partners with cytostatic proline rich polypeptide 1 in human chondrosarcoma. Int J Oncol 52:139-154 (2018). Read more (PubMed: 29138803) »
  • Moretti IF  et al. Plasmatic membrane toll-like receptor expressions in human astrocytomas. PLoS One 13:e0199211 (2018). Read more (PubMed: 29912993) »
See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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