Anti-TLR6 antibody [ABM1B50] (ab228424)

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Overview

  • Product name

    Anti-TLR6 antibody [ABM1B50]
    See all TLR6 primary antibodies

  • Description

    Mouse monoclonal [ABM1B50] to TLR6

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human TLR6 aa 280-512.
    Sequence:

    YLNIYNLTIIESIREEDFTYSKTTLKALTIEHITNQVFLFSQTALYTVFS EMNIMMLTISDTPFIHMLCPHAPSTFKFLNFTQNVFTDSIFEKCSTLVKL ETLILQKNGLKDLFKVGLMTKDMPSLEILDVSWNSLESGRHKENCTWVES IVVLNLSSNMLTDSVFRCLPPRIKVLDLHSNKIKSVPKQVVKLEALQELN VAFNSLTDLPGCGSFSSLSVL


    Database link: Q9Y2C9

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Jurkat whole cell lysate (ab7899). Flow Cyt: Jurkat cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab228424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.5-1µg for 106 cells.
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 92 kDa.

Target

  • Function

    Participates in the innate immune response to Gram-positive bacteria and fungi. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR2.

  • Tissue specificity

    Detected in monocytes, CD11c+ immature dendritic cells, plasmacytoid pre-dendritic cells and dermal microvessel endothelial cells.

  • Sequence similarities

    Belongs to the Toll-like receptor family.
    Contains 12 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.

  • Cellular localization

    Cell membrane. Cytoplasmic vesicle > phagosome membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD286 antibody
      • CD286 antigen antibody
      • TLR 6 antibody
      • Tlr6 antibody
      • TLR6_HUMAN antibody
      • Toll like receptor 6 precursor antibody
      • Toll-like receptor 6 antibody
      see all

    Images

    • Western blot - Anti-TLR6 antibody [ABM1B50] (ab228424)
      Western blot - Anti-TLR6 antibody [ABM1B50] (ab228424)
      Anti-TLR6 antibody [ABM1B50] (ab228424) at 2 µg/ml + Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate

      Predicted band size: 92 kDa

    • Flow Cytometry - Anti-TLR6 antibody [ABM1B50] (ab228424)
      Flow Cytometry - Anti-TLR6 antibody [ABM1B50] (ab228424)

      Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cell line labeling TLR6 with ab228424 at 0.5 µg/106 cells (red) compared with an isotype control of rabbit IgG (green). Goat anti-Mouse PE conjugate was used as secondary antibody.

    References

    ab228424 has not yet been referenced specifically in any publications.

    Publishing research using ab228424? Please let us know so that we can cite the reference in this datasheet.

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