Overview

  • Product name

    Anti-TLR7 antibody [4F4]
    See all TLR7 primary antibodies
  • Description

    Mouse monoclonal [4F4] to TLR7
  • Host species

    Mouse
  • Specificity

    ab28048 recognises TLR7.
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment purified from E. coli: SVESYEPQVL EQLHYFRYDK YARSCRFKNK EASFMSVNES CYKYGQTLDL, corresponding to amino acids 451/500 of Human TLR7

  • Positive control

    • Ramos cell lysates
  • General notes

    This product was changed from ascites to tissue culture supernatant on 28/02/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab28048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: recommended starting dilution: 1/1000.
    WB: 1/500 - 1/2000. Predicted molecular weight: 121 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
    • Tissue specificity

      Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells.
    • Sequence similarities

      Belongs to the Toll-like receptor family.
      Contains 27 LRR (leucine-rich) repeats.
      Contains 1 TIR domain.
    • Cellular localization

      Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
    • Information by UniProt
    • Database links

    • Alternative names

      • PRO285 antibody
      • TLR 7 antibody
      • Tlr7 antibody
      • TLR7_HUMAN antibody
      • Toll like receptor 7 antibody
      • Toll-like receptor 7 antibody
      • UNQ248 antibody
      see all

    Images

    • Anti-TLR7 antibody [4F4] (ab28048) at 1/1000 dilution + 80 µg Ramos cell lysate

      Secondary
      goat anti mouse secondary antibody conjugated to HRP

      Developed using the ECL technique.

      Predicted band size: 121 kDa
      Observed band size: 121 kDa



      This image was generated using the ascites version of the product.

    References

    This product has been referenced in:

    • Makni-Maalej K  et al. The TLR7/8 agonist CL097 primes N-formyl-methionyl-leucyl-phenylalanine-stimulated NADPH oxidase activation in human neutrophils: critical role of p47phox phosphorylation and the proline isomerase Pin1. J Immunol 189:4657-65 (2012). WB . Read more (PubMed: 23002436) »
    • Wang K  et al. Correlation of TLR1-10 expression in peripheral blood mononuclear cells with chronic hepatitis B and chronic hepatitis B-related liver failure. Hum Immunol 71:950-6 (2010). WB ; Human . Read more (PubMed: 20667457) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab28048.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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