Overview

  • Product name
    Anti-TLR7 antibody [4F4]
    See all TLR7 primary antibodies
  • Description
    Mouse monoclonal [4F4] to TLR7
  • Host species
    Mouse
  • Specificity
    ab28048 recognises TLR7.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment purified from E. coli: SVESYEPQVL EQLHYFRYDK YARSCRFKNK EASFMSVNES CYKYGQTLDL, corresponding to amino acids 451/500 of Human TLR7

  • Positive control
    • Ramos cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab28048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: recommended starting dilution: 1/1000.
    WB: 1/500 - 1/2000. Predicted molecular weight: 121 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
    • Tissue specificity
      Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells.
    • Sequence similarities
      Belongs to the Toll-like receptor family.
      Contains 27 LRR (leucine-rich) repeats.
      Contains 1 TIR domain.
    • Cellular localization
      Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
    • Information by UniProt
    • Database links
    • Alternative names
      • PRO285 antibody
      • TLR 7 antibody
      • Tlr7 antibody
      • TLR7_HUMAN antibody
      • Toll like receptor 7 antibody
      • Toll-like receptor 7 antibody
      • UNQ248 antibody
      see all

    Images

    • Anti-TLR7 antibody [4F4] (ab28048) at 1/1000 dilution + 80 µg Ramos cell lysate

      Secondary
      goat anti mouse secondary antibody conjugated to HRP

      Developed using the ECL technique.

      Predicted band size: 121 kDa
      Observed band size: 121 kDa

    References

    This product has been referenced in:
    • Makni-Maalej K  et al. The TLR7/8 agonist CL097 primes N-formyl-methionyl-leucyl-phenylalanine-stimulated NADPH oxidase activation in human neutrophils: critical role of p47phox phosphorylation and the proline isomerase Pin1. J Immunol 189:4657-65 (2012). WB . Read more (PubMed: 23002436) »
    • Wang K  et al. Correlation of TLR1-10 expression in peripheral blood mononuclear cells with chronic hepatitis B and chronic hepatitis B-related liver failure. Hum Immunol 71:950-6 (2010). WB ; Human . Read more (PubMed: 20667457) »
    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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