Anti-TLR7 antibody (ab24184)

Submit a review Q&A (2)References (2)

Key features and details

  • Rabbit polyclonal to TLR7
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-TLR7 antibody
    See all TLR7 primary antibodies

  • Description

    Rabbit polyclonal to TLR7

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide (Mouse) corresponding to 14 amino acids near the middle of mouse TLR7.

  • Positive control

    • Daudi cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab24184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 2 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 122 kDa.

Target

  • Function

    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.

  • Tissue specificity

    Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells.

  • Sequence similarities

    Belongs to the Toll-like receptor family.
    Contains 27 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.

  • Cellular localization

    Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
  • Information by UniProt

  • Database links

    • Alternative names

      • PRO285 antibody
      • TLR 7 antibody
      • Tlr7 antibody
      • TLR7_HUMAN antibody
      • Toll like receptor 7 antibody
      • Toll-like receptor 7 antibody
      • UNQ248 antibody
      see all

    Images

    • Western blot - Anti-TLR7 antibody (ab24184)
      Western blot - Anti-TLR7 antibody (ab24184)
      Lane 1 : Anti-TLR7 antibody (ab24184) at 0.5 µg/ml
      Lane 2 : Anti-TLR7 antibody (ab24184) at 1 µg/ml
      Lane 3 : Anti-TLR7 antibody (ab24184) at 2 µg/ml

      All lanes : Daudi cell lysates.

      Predicted band size: 122 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-TLR7 antibody (ab24184)
      Immunocytochemistry/ Immunofluorescence - Anti-TLR7 antibody (ab24184)

      ab24184 at 2µg/ml staining of TLR7 in Daudi cells by ICC/IF

    References

    Publishing research using ab24184? Please let us know so that we can cite the reference in this datasheet.

    ab24184 has been referenced in 2 publications.

    • Hu Y  et al. The miR-122 inhibition alleviates lipid accumulation and inflammation in NAFLD cell model. Arch Physiol Biochem N/A:1-5 (2019). PubMed: 31311339
    • Delgado MA  et al. Toll-like receptors control autophagy. EMBO J 27:1110-21 (2008). WB ; Mouse . PubMed: 18337753

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    Protocole (Westernblot)
    1. Concentration/dilution de l'anticorps:
    1/500 (2ug/ml) diluted in agent bloquant (lait/BSA 5%, sélectionnés dépendant un anticorps anti-TLR3 chez vous -ab12085 qui fonctionne bien)
    Incubation à 4 dégrées overnight.
    Incubation avec un secondaire-HRP contre l'espèce qui fabrique l'anticorps 1hr à température ambiante

    2. Préparation des échantillons:
    Lyse en utilisant la solution classiques pour TLR3 (TLR endomosal).

    3. Pour un contrôle positif (car on n'a pas de signal, le contrôle négatifs n'est pas pris en compte), j'ai utilisé les lymphocytes T immortalisé qui expriment ces récepteurs TLR (3, 4, 7, 9).

    Je vous envoie une photo de la membrane qui révèle rien de la protéine TLR7 (La 3ème ligne est la révélation de IkB-a.)

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    Answer

    Merci d'avoir pris le temps de remplir notre petitquestionnaire et de nous avoir contactés.

    Je suis désolé d'apprendre que vous avez eu des difficultés à obtenir de bons résultats avec ab24184.

    Je pense que malheureusement vous avez reçu un tube ou un lot défectueux.Nous n'avons enstock que le lotque vous avez reçu, j'aimeraisdonc vous proposer en compensationun avoir, un remboursement ouun remplacement gratuitavec uneréférence produit alternative de la liste suivante(en excluant bien sur le ab24184) :https://www.abcam.com/index.html?pageconfig=searchresults&search=TLR7&pt=1&sk=app&sv=69&sn=WB&l=1&fViewMore=1

    Merci de m'indiquerlarésolution quivous conviendrait le mieux.

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    Question

    Details of how this antibody was tested. And specificity determined

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    Answer

    Thank you for your enquiry. I have been in touch with the source of this antibody to determine how it was tested. I can tell you that this antibody was assayed using a panel of tissues from human, mouse, and rat. Tissue lysates with bands at the appropriate molecular weight were deemed as positive results. A peptide blocking study was also performed with the serum in A20 and Daudi cell lysates. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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