Overview

  • Product name
  • Description
    Rabbit polyclonal to TLR7
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (Mouse) corresponding to 14 amino acids near the middle of mouse TLR7.

  • Positive control
    • Daudi cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab24184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 2 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 122 kDa.

Target

  • Function
    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificity
    Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells.
  • Sequence similarities
    Belongs to the Toll-like receptor family.
    Contains 27 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • Cellular localization
    Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
  • Information by UniProt
  • Database links
  • Alternative names
    • PRO285 antibody
    • TLR 7 antibody
    • Tlr7 antibody
    • TLR7_HUMAN antibody
    • Toll like receptor 7 antibody
    • Toll-like receptor 7 antibody
    • UNQ248 antibody
    see all

Images

  • Lane 1 : Anti-TLR7 antibody (ab24184) at 0.5 µg/ml
    Lane 2 : Anti-TLR7 antibody (ab24184) at 1 µg/ml
    Lane 3 : Anti-TLR7 antibody (ab24184) at 2 µg/ml

    All lanes : Daudi cell lysates.

    Predicted band size: 122 kDa

  • ab24184 at 2µg/ml staining of TLR7 in Daudi cells by ICC/IF

References

This product has been referenced in:
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Merci d'avoir pris le temps de remplir notre petitquestionnaire et de nous avoir contactés.

Je suis désolé d'apprendre que vous avez eu des difficultés à obtenir de bons résultats avec ab24184.

Je pense que malheureusement vous avez reçu un tube ou un lot défectueux.Nous n'avons enstock que le lotque vous avez reçu, j'aimeraisdonc vous proposer en compensationun avoir, un remboursement ouun remplacement gratuitavec uneréférence produit alternative de la liste suivante(en excluant bien sur le ab24184) :https://www.abcam.com/index.html?pageconfig=searchresults&search=TLR7&pt=1&sk=app&sv=69&sn=WB&l=1&fViewMore=1

Merci de m'indiquerlarésolution quivous conviendrait le mieux.

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Question
Answer

Thank you for your enquiry. I have been in touch with the source of this antibody to determine how it was tested. I can tell you that this antibody was assayed using a panel of tissues from human, mouse, and rat. Tissue lysates with bands at the appropriate molecular weight were deemed as positive results. A peptide blocking study was also performed with the serum in A20 and Daudi cell lysates. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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