Overview

  • Product name
    Anti-TLR7 antibody
    See all TLR7 primary antibodies
  • Description
    Rabbit polyclonal to TLR7
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (Mouse) corresponding to 14 amino acids near the middle of mouse TLR7.

  • Positive control
    • Daudi cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab24184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 2 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 122 kDa.

Target

  • Function
    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificity
    Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells.
  • Sequence similarities
    Belongs to the Toll-like receptor family.
    Contains 27 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • Cellular localization
    Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
  • Information by UniProt
  • Database links
    • Alternative names
      • PRO285 antibody
      • TLR 7 antibody
      • Tlr7 antibody
      • TLR7_HUMAN antibody
      • Toll like receptor 7 antibody
      • Toll-like receptor 7 antibody
      • UNQ248 antibody
      see all

    Images

    • Western blot - Anti-TLR7 antibody (ab24184)
      Western blot - Anti-TLR7 antibody (ab24184)
      Lane 1 : Anti-TLR7 antibody (ab24184) at 0.5 µg/ml
      Lane 2 : Anti-TLR7 antibody (ab24184) at 1 µg/ml
      Lane 3 : Anti-TLR7 antibody (ab24184) at 2 µg/ml

      All lanes : Daudi cell lysates.

      Predicted band size: 122 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-TLR7 antibody (ab24184)
      Immunocytochemistry/ Immunofluorescence - Anti-TLR7 antibody (ab24184)

      ab24184 at 2µg/ml staining of TLR7 in Daudi cells by ICC/IF

    References

    This product has been referenced in:

    See 1 Publication for this product

    Publishing research using ab24184? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    2 reviews or Q&A

    10
    Votes: Highest First

    Protocole (Westernblot)
    1. Concentration/dilution de l'anticorps:
    1/500 (2ug/ml) diluted in agent bloquant (lait/BSA 5%, sélectionnés dépendant un anticorps anti-TLR3 chez vous -ab12085 qui fonctionne bien)
    Incubatio...

    Read More

    Merci d'avoir pris le temps de remplir notre petitquestionnaire et de nous avoir contactés.

    Je suis désolé d'apprendre que vous avez eu des difficultés à obtenir de bons résultats avec ab24184.
    ...

    Read More

    Details of how this antibody was tested. And specificity determined

    Thank you for your enquiry. I have been in touch with the source of this antibody to determine how it was tested. I can tell you that this antibody was assayed using a panel of tissues from human, mouse, and rat. Tissue lysates with bands at the app...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up