Recombinant Anti-TLR7 antibody [EPR2088(2)] (ab124928)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2088(2)] to TLR7
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TLR7 antibody [EPR2088(2)]
See all TLR7 primary antibodies -
Description
Rabbit monoclonal [EPR2088(2)] to TLR7 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human TLR7 aa 1000 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q9NYK1 -
Positive control
- WB: Raji, Daudi and Ramos cell lysates. IHC-P: Human tonsil tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 2.90 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2088(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124928 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/1000. Detects a band of approximately 140 kDa (predicted molecular weight: 121 kDa).
For unpurified use at 1/300. To achieve desired WB results, do not to boil samples after lysis. |
IHC-P | (3) |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/30. |
Notes |
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WB
1/1000. Detects a band of approximately 140 kDa (predicted molecular weight: 121 kDa). For unpurified use at 1/300. To achieve desired WB results, do not to boil samples after lysis. |
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/30. |
Target
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Function
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
Tissue specificity
Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 27 LRR (leucine-rich) repeats.
Contains 1 TIR domain. -
Cellular localization
Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist. - Information by UniProt
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Database links
- Entrez Gene: 51284 Human
- Omim: 300365 Human
- SwissProt: Q9NYK1 Human
- Unigene: 659215 Human
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Alternative names
- PRO285 antibody
- TLR 7 antibody
- Tlr7 antibody
see all
Images
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All lanes : Anti-TLR7 antibody [EPR2088(2)] (ab124928) at 1/1000 dilution
Lane 1 : Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate (boiled)
Lane 2 : Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate (unboiled)
Lane 3 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate (boiled)
Lane 4 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate (unboiled)
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 121 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsBlocking and diluting buffer and concentration: 5% NFDM /TBST.
We recommend not boiling the samples after lysis to get desired WB results.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TLR7 with unpurified ab124928 at 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TLR7 with purified ab124928 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TLR7 with unpurified ab124928 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab124928 has been referenced in 14 publications.
- Zhang Y et al. Triptolide in the treatment of systemic lupus erythematosus - regulatory effects on miR-146a in B cell TLR7 signaling pathway in mice. Front Pharmacol 13:952775 (2022). PubMed: 36210830
- Bouvet M et al. Multiple Viral microRNAs Regulate Interferon Release and Signaling Early during Infection with Epstein-Barr Virus. mBio 12:N/A (2021). PubMed: 33785626
- Proskocil BJ et al. TLR7 is expressed by support cells, but not sensory neurons, in ganglia. J Neuroinflammation 18:209 (2021). PubMed: 34530852
- Zheng N et al. TLR7 in B cells promotes renal inflammation and Gd-IgA1 synthesis in IgA nephropathy. JCI Insight 5:N/A (2020). PubMed: 32699192
- Azar P et al. TLR7 dosage polymorphism shapes interferogenesis and HIV-1 acute viremia in women. JCI Insight 5:N/A (2020). WB . PubMed: 32554924