Overview

  • Product name

    Anti-TLR7 antibody [EPR2088(2)]
    See all TLR7 primary antibodies
  • Description

    Rabbit monoclonal [EPR2088(2)] to TLR7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TLR7 aa 1000 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q9NYK1

  • Positive control

    • WB: Raji, Daudi and Ramos cell lysates. IHC-P: Human tonsil tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 140 kDa (predicted molecular weight: 121 kDa).

For unpurified use at 1/300.

IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/30.

  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
    • Tissue specificity

      Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells.
    • Sequence similarities

      Belongs to the Toll-like receptor family.
      Contains 27 LRR (leucine-rich) repeats.
      Contains 1 TIR domain.
    • Cellular localization

      Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
    • Information by UniProt
    • Database links

    • Alternative names

      • PRO285 antibody
      • TLR 7 antibody
      • Tlr7 antibody
      • TLR7_HUMAN antibody
      • Toll like receptor 7 antibody
      • Toll-like receptor 7 antibody
      • UNQ248 antibody
      see all

    Images

    • All lanes : Anti-TLR7 antibody [EPR2088(2)] (ab124928) at 20 µg (unpurified)

      Lane 1 : Ramos cell lysate
      Lane 2 : Daudi cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution

      Predicted band size: 121 kDa
      Observed band size: 140 kDa
      why is the actual band size different from the predicted?



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TLR7 with unpurified ab124928 at 1/100.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TLR7 with purified ab124928 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.

    • All lanes : Anti-TLR7 antibody [EPR2088(2)] (ab124928) (purified)

      Lane 1 : Ramos cell lysate
      Lane 2 : Daudi cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 121 kDa
      Observed band size: 140 kDa why is the actual band size different from the predicted?



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-TLR7 antibody [EPR2088(2)] (ab124928) at 1/1000 dilution (unpurified)

      Lane 1 : Raji cell lysates
      Lane 2 : Ramos cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution

      Predicted band size: 121 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TLR7 with unpurified ab124928 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Souyris M  et al. TLR7 escapes X chromosome inactivation in immune cells. Sci Immunol 3:N/A (2018). Read more (PubMed: 29374079) »
    • Wang Y  et al. Increased infiltration of CD11 c+/CD123+ dendritic cell subsets and upregulation of TLR/IFN-a signaling participate in pathogenesis of oral lichen planus. Oral Surg Oral Med Oral Pathol Oral Radiol 125:459-467.e2 (2018). IHC-P . Read more (PubMed: 29429903) »
    See all 6 Publications for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rhesus monkey Tissue sections (macaque retina)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate pH 6
    Permeabilization
    No
    Specification
    macaque retina
    Blocking step
    Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Mar 14 2017

    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (8)
    Sample
    Human Tissue lysate - whole (Heart)
    Specification
    Heart
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Nov 14 2013

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Sample
    Human Tissue sections (Spleen)
    Specification
    Spleen
    Permeabilization
    No
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Nov 12 2013

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (peripheral blood cells)
    Loading amount
    30 µg
    Specification
    peripheral blood cells
    Gel Running Conditions
    Reduced Denaturing (MES buffer, 4-12% Bis-Tris Gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Sep 17 2012

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