Key features and details
- Mouse monoclonal [44C143] to TLR8
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG1
Product nameAnti-TLR8 antibody [44C143]
See all TLR8 primary antibodies
DescriptionMouse monoclonal [44C143] to TLR8
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide conjugated to KLH, corresponding to a region within amino acids 750-850 of Human TLR8
- Human brain cortex, Human spleen, 293 transfected with human TLR8, and Ramos cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.005% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab85859 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 120 kDa.|
|IHC-P||Use a concentration of 5 µg/ml.|
|Flow Cyt||Use 0.5µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionKey component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
Tissue specificityDetected in brain, heart, lung, liver, placenta, in monocytes, and at lower levels in CD11c+ immature dendritic cells.
Sequence similaritiesBelongs to the Toll-like receptor family.
Contains 23 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain.
- Information by UniProt
- CD 288 antibody
- CD288 antibody
- CD288 antigen antibody
ab85859 at 0.5µg in 1x106 Ramos cells
Shaded histogram represents Ramos cells without antibody; green represents isotype control; purple represents TLR8 antibody.
ab85859 at 5 µg/ml staining TLR8 in formalin fixed and paraffin embedded human spleen tissue
Anti-TLR8 antibody [44C143] (ab85859) at 2 µg/ml + 293 cell lysate transfected with Human TLR8
Predicted band size: 120 kDa
Observed band size: 120 kDa
ab85859 has been referenced in 2 publications.
- Ahmad R et al. Increased adipose tissue expression of TLR8 in obese individuals with or without type-2 diabetes: significance in metabolic inflammation. J Inflamm (Lond) 13:38 (2016). PubMed: 27980459
- Li Z et al. Inhibition of IRAK1/4 sensitizes T cell acute lymphoblastic leukemia to chemotherapies. J Clin Invest 125:1081-97 (2015). PubMed: 25642772