• Product name
  • Description
    Rabbit polyclonal to TLR8
  • Host species
  • Specificity
    ab24185 reacts with TLR8 protein.
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (Human) corresponding to 16 amino acids near the middle of human TLR8 (within the Leucine-rich repeat between residues 772 and 823).

  • Positive control
    • Daudi cell lysate.



Our Abpromise guarantee covers the use of ab24185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 2 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 119 kDa.


  • Function
    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificity
    Detected in brain, heart, lung, liver, placenta, in monocytes, and at lower levels in CD11c+ immature dendritic cells.
  • Sequence similarities
    Belongs to the Toll-like receptor family.
    Contains 23 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 288 antibody
    • CD288 antibody
    • CD288 antigen antibody
    • MGC119599 antibody
    • MGC119600 antibody
    • TLR 8 antibody
    • Tlr8 antibody
    • TLR8_HUMAN antibody
    • Toll like receptor 8 antibody
    • Toll-like receptor 8 antibody
    see all


  • Lane 1 : Anti-TLR8 antibody (ab24185) at 0.5 µg/ml
    Lane 2 : Anti-TLR8 antibody (ab24185) at 1 µg/ml
    Lane 3 : Anti-TLR8 antibody (ab24185) at 2 µg/ml

    All lanes : Daudi Cell lysate

    Predicted band size: 119 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?

  • ab25185 at 2µg/ml staining TLR8 in Daudi cells by ICC/IF


This product has been referenced in:
  • Yu L  et al. Functional Roles of Pattern Recognition Receptors That Recognize Virus Nucleic Acids in Human Adipose-Derived Mesenchymal Stem Cells. Biomed Res Int 2016:9872138 (2016). Read more (PubMed: 28105439) »
  • Umiker BR  et al. Dosage of X-linked Toll-like receptor 8 determines gender differences in the development of systemic lupus erythematosus. Eur J Immunol 44:1503-16 (2014). Read more (PubMed: 24500834) »
See all 7 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A


Thank you for your reply.

I'm sending a free of charge vial of ab95047 on the order ***, which should arrive tomorrow.

Please keep me updated about the results using this replacement antibody, and let me know if there is anything else that we can do for you.

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Thank you for your call today and for letting us know about the trouble with ab24185 in Western blot.

As we discussed, I would recommend checking the membrane with Ponceau stain to make sure that the protein transferred, however I would be happy to send a replacement antibody as the band around 50-60 kDa seems to be non-specific.

Please let me know if there is an antibody that you would like to received as a replacement, and I will send it out promptly.

I look forward to hearing from you. Let me know if you have any questions or if there is anything else that we can do for you.

Read More


The peptide was selected from somewhere within the Leucine-rich repeat between residues 772 and 823. Unfortunately, I can not get more specific than that but I hope this will help you, Please do not hesitate to contact us again if you need further information,

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