Anti-TLR8 antibody [OTI4F3] (ab236521)
Key features and details
- Mouse monoclonal [OTI4F3] to TLR8
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-TLR8 antibody [OTI4F3]
See all TLR8 primary antibodies -
Description
Mouse monoclonal [OTI4F3] to TLR8 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human TLR8 aa 32-153. (NP_619542) produced in E.coli.
Database link: Q9NR97 -
Positive control
- IHC-P: Human spleen and spleen carcinoma tissue. WB: pCMV6-ENTRY TLR8 cDNA transfected HEK-293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
OTI4F3 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236521 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000. Predicted molecular weight: 120 kDa.
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IHC-P |
1/2000.
Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 minutes. |
Notes |
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WB
1/2000. Predicted molecular weight: 120 kDa. |
IHC-P
1/2000. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 minutes. |
Target
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Function
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
Tissue specificity
Detected in brain, heart, lung, liver, placenta, in monocytes, and at lower levels in CD11c+ immature dendritic cells. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 23 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 51311 Human
- Omim: 300366 Human
- SwissProt: Q9NR97 Human
- Unigene: 660543 Human
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Alternative names
- CD 288 antibody
- CD288 antibody
- CD288 antigen antibody
see all
Images
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Paraffin-embedded human spleen tissue stained for TLR8 using ab236521 at 1/2000 dilution in immunohistochemical analysis.
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All lanes : Anti-TLR8 antibody [OTI4F3] (ab236521) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY TLR8 cDNA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 120 kDa -
Paraffin-embedded human spleen carcinoma tissue stained for TLR8 using ab236521 at 1/2000 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab236521 has not yet been referenced specifically in any publications.