Overview

  • Product name

    Anti-TLR8 antibody [OTI4F3]
    See all TLR8 primary antibodies
  • Description

    Mouse monoclonal [OTI4F3] to TLR8
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human TLR8 aa 32-153. (NP_619542) produced in E.coli.
    Database link: Q9NR97

  • Positive control

    • IHC-P: Human spleen and spleen carcinoma tissue. WB: pCMV6-ENTRY TLR8 cDNA transfected HEK-293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab236521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 120 kDa.
IHC-P 1/2000.

Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 minutes.

Target

  • Function

    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificity

    Detected in brain, heart, lung, liver, placenta, in monocytes, and at lower levels in CD11c+ immature dendritic cells.
  • Sequence similarities

    Belongs to the Toll-like receptor family.
    Contains 23 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 288 antibody
    • CD288 antibody
    • CD288 antigen antibody
    • MGC119599 antibody
    • MGC119600 antibody
    • TLR 8 antibody
    • Tlr8 antibody
    • TLR8_HUMAN antibody
    • Toll like receptor 8 antibody
    • Toll-like receptor 8 antibody
    see all

Images

  • All lanes : Anti-TLR8 antibody [OTI4F3] (ab236521) at 1/2000 dilution

    Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 2 : pCMV6-ENTRY TLR8 cDNA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 120 kDa

  • Paraffin-embedded human spleen tissue stained for TLR8 using ab236521 at 1/2000 dilution in immunohistochemical analysis.

  • Paraffin-embedded human spleen carcinoma tissue stained for TLR8 using ab236521 at 1/2000 dilution in immunohistochemical analysis.

References

ab236521 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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