Key features and details
- Mouse monoclonal [OTI4F3] to TLR8
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-TLR8 antibody [OTI4F3]
See all TLR8 primary antibodies
DescriptionMouse monoclonal [OTI4F3] to TLR8
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human TLR8 aa 32-153. (NP_619542) produced in E.coli.
Database link: Q9NR97
- IHC-P: Human spleen and spleen carcinoma tissue. WB: pCMV6-ENTRY TLR8 cDNA transfected HEK-293T cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purification notesPurified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab236521 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 120 kDa.|
Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 minutes.
FunctionKey component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
Tissue specificityDetected in brain, heart, lung, liver, placenta, in monocytes, and at lower levels in CD11c+ immature dendritic cells.
Sequence similaritiesBelongs to the Toll-like receptor family.
Contains 23 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain.
- Information by UniProt
- CD 288 antibody
- CD288 antibody
- CD288 antigen antibody
Paraffin-embedded human spleen tissue stained for TLR8 using ab236521 at 1/2000 dilution in immunohistochemical analysis.
All lanes : Anti-TLR8 antibody [OTI4F3] (ab236521) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY TLR8 cDNA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 120 kDa
Paraffin-embedded human spleen carcinoma tissue stained for TLR8 using ab236521 at 1/2000 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236521 has not yet been referenced specifically in any publications.