Anti-TLR9 antibody [26C593.2] (ab134368)
Key features and details
- Mouse monoclonal [26C593.2] to TLR9
- Suitable for: WB, IHC-Fr, Flow Cyt (Intra), ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-TLR9 antibody [26C593.2]
See all TLR9 primary antibodies -
Description
Mouse monoclonal [26C593.2] to TLR9 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Fr, Flow Cyt (Intra), ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chimpanzee, Cynomolgus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human TLR9 aa 268-284 conjugated to keyhole limpet haemocyanin. AAF78037
Sequence:CPRHFPQLHPDTFSHLS
Database link: Q9NR96 -
Positive control
- WB: Human peripheral blood mononuclear cells (PBMCs). Human, mouse and rat intestine tissue lysates. Flow Cyt (Intra): Ramos and PBMC cells. IHC-Fr: Human lung adenocarcinoma and malignant lung tissues. IHC-P: Human spleen tissue. ICC/IF: A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (up to 6 months). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
26C593.2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134368 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 115 kDa).
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IHC-Fr |
Use a concentration of 10 - 20 µg/ml.
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Flow Cyt (Intra) |
Use 0.1-2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
Use a concentration of 5 µg/ml.
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Notes |
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WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 115 kDa). |
IHC-Fr
Use a concentration of 10 - 20 µg/ml. |
Flow Cyt (Intra)
Use 0.1-2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 5 µg/ml. |
Target
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Function
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
Tissue specificity
Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 26 LRR (leucine-rich) repeats.
Contains 1 TIR domain. -
Cellular localization
Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist. - Information by UniProt
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Database links
- Entrez Gene: 54106 Human
- Entrez Gene: 81897 Mouse
- Entrez Gene: 338457 Rat
- Omim: 605474 Human
- SwissProt: Q9NR96 Human
- SwissProt: Q9EQU3 Mouse
- Unigene: 87968 Human
- Unigene: 44889 Mouse
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Alternative names
- CD 289 antibody
- CD289 antibody
- TLR 9 antibody
see all
Images
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Flow Cytometric analysis of TLR9 in Ramos (Human Burkitt's lymphoma cell line) cells labelled with ab134368 at 0.1 µg (pink), no antibody (blue) or isotype control antibody (green). Goat anti-mouse IgG FITC secondary was used.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR9 antibody [26C593.2] (ab134368)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling TLR9 with ab134368 at 5 µg/ml.
Staining was enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.
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All lanes : Anti-TLR9 antibody [26C593.2] (ab134368) at 3 µg/ml
Lane 1 : Human peripheral blood mononuclear cells (PBMCs)
Lane 2 : Human intestine tissue lysate
Lane 3 : Mouse intestine tissue lysate
Lane 4 : Rat intestine tissue lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat anti-mouse HRP conjugate
Predicted band size: 115 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted? -
Flow Cytometric analysis of TLR9 in human PBMC cells labelled with ab134368 at 0.5 µg (red) or an isotype control antibody (green).
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Immunohistochemical analysis of TLR9 in frozen sections of human lung adenocarcinoma tissue, using ab134368 at a 1/100 dilution.
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Immunohistochemical analysis of TLR9 in frozen sections of A549 (Human lung carcinoma cell line) cells, using ab134368 at a 1/100 dilution.
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Immunohistochemical analysis of TLR9 in frozen sections of malignant human lung tissue, using ab134368 at a 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab134368 has been referenced in 11 publications.
- Ishikawa T et al. Chronic Inhibition of Toll-Like Receptor 9 Ameliorates Pulmonary Hypertension in Rats. J Am Heart Assoc 10:e019247 (2021). PubMed: 33787285
- Olejarz W et al. TLRs and RAGE are elevated in carotid plaques from patients with moderate-to-severe obstructive sleep apnea syndrome. Sleep Breath 24:1573-1580 (2020). PubMed: 32076951
- Luo Q et al. TLR9 induces colitis-associated colorectal carcinogenesis by regulating NF-?B expression levels. Oncol Lett 20:110 (2020). PubMed: 32863923
- Qian J et al. High expression levels of TLR9 and PD-L1 indicates a poor prognosis in patients with angioimmunoblastic T-cell lymphoma: a retrospective study of 88 cases in a single center. J Cancer 11:57-68 (2020). PubMed: 31892973
- Zhang S et al. HMGB1/RAGE axis mediates stress-induced RVLM neuroinflammation in mice via impairing mitophagy flux in microglia. J Neuroinflammation 17:15 (2020). PubMed: 31924219