• Product name
    Anti-TLR9 antibody [5G5]
    See all TLR9 primary antibodies
  • Description
    Mouse monoclonal [5G5] to TLR9
  • Host species
  • Specificity
    This antibody reacts very well in mouse, but more weakly in human. There are references available which suggest the antibody works well in human, however, customer feedback suggests results in human can be inconsistant.
  • Tested applications
    Suitable for: IHC-P, Flow Cyt, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Dog
    Does not react with: Human
  • Immunogen

    Purified fusion protein of extracellular domain of human TLR9 (AA 1-815) and human IgGFc.

  • Positive control
    • RAW macrophages



Our Abpromise guarantee covers the use of ab16892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: 1/50.
    IHC-Fr: 1/50.Ab16892 stains RAW macrophages and TLR9 transfected HEK293 cells.
    IHC-P: Use at an assay dependent dilution.
    WB: 1/50. Predicted molecular weight: 116 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
    • Tissue specificity
      Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.
    • Sequence similarities
      Belongs to the Toll-like receptor family.
      Contains 26 LRR (leucine-rich) repeats.
      Contains 1 TIR domain.
    • Cellular localization
      Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 289 antibody
      • CD289 antibody
      • TLR 9 antibody
      • TLR9 antibody
      • TLR9_HUMAN antibody
      • Toll like receptor 9 antibody
      • Toll like receptor 9 isoform A precursor antibody
      • Toll like receptor 9 isoform B antibody
      • Toll-like receptor 9 antibody
      see all


    This product has been referenced in:
    • Machida H  et al. Expression of Toll-like receptor 9 in renal podocytes in childhood-onset active and inactive lupus nephritis. Nephrol Dial Transplant 25:2530-537 (2010). Read more (PubMed: 20181802) »
    • Ilversaro et al Toll like receptor 9 agonists stimulate prostate cancer invasion in vitro The Prostate 67:774-781 (2007). Read more (PubMed: ) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A


    Thank you for your inquiry.

    The products ab16892 and ab58864 are both from the same clone [5G5] and have the same immunogen: Purified fusion protein of extracellular domain of human TLR9 (AA 1-815) and human IgGFc.The immunogen for both is as described in the paper below:

    Ahmad-Nejad, P et al; Bacterial CpG-DNA and lipopolysaccharides activate Toll-like receptors at distinct cellular compartments. Eur J Immunol 2002, 32: 1958

    Regarding ab72362, the immunogen is BALB/c mice immunized with Ba/F3 cells that stably expressed human Myc-tagged TLR6. The epitope has not been mapped.
    I hope this information helps. Please contact us with any other questions.

    Read More


    Thank you very much for your interest in our products.

    To our knowledge,none of our TLR6 or TLR9 antibodies havebeen tested in bovine. Since we have over 90,000 products we cannot store multiple vial sizes for testing. Therefore, I can offer a discount off a future purchase if you buyanti-TLR6 and anti-TLR9antibodies now, test them inbovine and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody per antibody tested.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceedand let me know the catalogue number of the product/s youwould like to testin bovine. I will then send thediscount code/s.The codesmust be issued before purchasing the antibodies so please wait for my reply before ordering.

    2. Purchasethe antibodyeither by phone, fax, or online (www.abcam.com).

    3. Test it in bovine.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.
    Please remember that submission of the Abreview is sufficient for the discount code to become active.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even ifthe antibodyturns out to be unsuitable for bovine, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.

    Read More
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Human Tissue sections (pancreatic)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: DAKO antigen retrieval buffer pH9

    Dr. Ilka Werner-Martini

    Verified customer

    Submitted Oct 12 2010


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