Product nameAnti-TLR9 antibody [5G5] (Biotin)
See all TLR9 primary antibodies
DescriptionMouse monoclonal [5G5] to TLR9 (Biotin)
Tested applicationsSuitable for: IHC-P, ICC/IF, Flow Cyt, WB, IHC-Frmore details
Species reactivityReacts with: Mouse, Dog
Does not react with: Human
Purified fusion protein of the extracellular domain of human TLR9 (AA 1-815) and human IgGFc
- RAW macrophages and TLR9 transfected HEK293 cells. RAW264.7 macrophages stimulated with IFN gamma.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA
Concentration information loading...
Purification notes0.2 µm filtered
Our Abpromise guarantee covers the use of ab17236 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab97679 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 115 kDa.|
|IHC-Fr||Use at an assay dependent concentration.|
FunctionKey component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
Tissue specificityHighly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.
Sequence similaritiesBelongs to the Toll-like receptor family.
Contains 26 LRR (leucine-rich) repeats.
Contains 1 TIR domain.
Cellular localizationEndoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
- Information by UniProt
- CD 289 antibody
- CD289 antibody
- TLR 9 antibody
Flow cytometry analysis of RAW264.7 cells, staining TLR9 with ab17236.
Cells were either untreated (green line) or treated (pink line) with GO5 or GO100 and fixed and permeabilized with 4% formaldehyde and 0.5% Tween-20. Cells were then incubated with primary antibody (1/100 dilution) for 1 hour at 4°C in the dark. An AlexaFluor®488-conjugated goat anti-mouse IgG was used as the secondary antibody.
Lane 1 : Anti-TLR9 antibody [5G5] (Biotin) (ab17236) at 1/500 dilution
Lane 2 : TLR9 Knockout
All lanes : Mouse liver whole tissue lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 115 kDa
Exposure time: 40 seconds
Blocking agent: 5% Milk for 1 hour at 23°C.
Immunfluorescence analysis of RAW264.7 cells, staining TLR9 with ab17236.
Cells were untreated (Un) or treated with GO5 or GO100 for 24 hours, fixed and then permeabilized. Cells were incubated with primary antibody (1/100) for for 1 hour at 4°C in the dark. An AlexaFluor®488-conjugated goat anti-mouse IgG was used as the secondary antibody.
This product has been referenced in:
- Chen GY et al. Simultaneous induction of autophagy and toll-like receptor signaling pathways by graphene oxide. Biomaterials 33:6559-69 (2012). ICC/IF, Flow Cyt ; Mouse . Read more (PubMed: 22704844) »
- Chen GY et al. Baculovirus transduction of mesenchymal stem cells triggers the toll-like receptor 3 pathway. J Virol 83:10548-56 (2009). Flow Cyt ; Human . Read more (PubMed: 19656899) »