• Product name
    Anti-TLR9 antibody [5G5] (FITC)
    See all TLR9 primary antibodies
  • Description
    Mouse monoclonal [5G5] to TLR9 (FITC)
  • Host species
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: IHC-P, WB, IHC-Fr, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Dog, Human
  • Immunogen

    Purified fusion protein of extracellular domain of human TLR9 (AA 1-815) and human IgGFc

  • Positive control
    • RAW macrophages stimulated with IFN-gamma



Our Abpromise guarantee covers the use of ab58864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50.
WB 1/50. Predicted molecular weight: 115 kDa.
IHC-Fr 1/50.
Flow Cyt 1/10 - 1/50.

ab91362 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


ICC/IF 1/50.


  • Function
    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificity
    Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.
  • Sequence similarities
    Belongs to the Toll-like receptor family.
    Contains 26 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • Cellular localization
    Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 289 antibody
    • CD289 antibody
    • TLR 9 antibody
    • TLR9 antibody
    • TLR9_HUMAN antibody
    • Toll like receptor 9 antibody
    • Toll like receptor 9 isoform A precursor antibody
    • Toll like receptor 9 isoform B antibody
    • Toll-like receptor 9 antibody
    see all


  • THP1 cells were incubated with an IgG2a isotype control (A) or a-TLR9 5G5 mAb (B). Cells (140000) were permeabilized with saponin and stained with 0.4 µg 5G5.


This product has been referenced in:
  • Dias AA  et al. DNA Sensing via TLR-9 Constitutes a Major Innate Immunity Pathway Activated during Erythema Nodosum Leprosum. J Immunol 197:1905-13 (2016). Read more (PubMed: 27474073) »
  • Ray A  et al. A novel TLR-9 agonist C792 inhibits plasmacytoid dendritic cell-induced myeloma cell growth and enhance cytotoxicity of bortezomib. Leukemia N/A:N/A (2014). Human . Read more (PubMed: 24476765) »

See all 3 Publications for this product

Customer reviews and Q&As

Merci pour votre réponse.
Comme convenu j'ai mis en place l'envoi d'une unité de ab134369 en remplacement du ab58864. Le numéro de commande de remplacement gratuit de ce produit est : ******.
Vous recevrez prochainement un mail de confirmatio...

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Merci de nous avoir contactés.
Nous sommes désolés d'apprendre que le produit que vous avez reçu ne fonctionne pas comme attendu.
Merci pour le partage de vos résultats et de votre protocole, ces informations sont très importantes pour nous c...

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Thank you for your inquiry.

The products ab16892 and ab58864 are both from the same clone [5G5] and have the same immunogen: Purified fusion protein of extracellular domain of human TLR9 (AA 1-815) and human IgGFc.The immunogen for both is as...

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Thank you very much for your interest in our products.

To our knowledge,none of our TLR6 or TLR9 antibodies havebeen tested in bovine. Since we have over 90,000 products we cannot store multiple vial sizes for testing. Therefore, I can offer ...

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Thank you for your reply.

Although the exact amount of FITC in ab58864 and ab81197 has not been determined, for the purposes of your calculations, you can assume that it is 40x greater.

Both ab16892 and ab58864, are the same clone o...

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Thank you for contacting us.

Ab81197 Mouse IgG2a (FITC) - Isotype control is supplied in a500 µl size at 4mg/ml. To correctly use this control you will need to use this at the same concentration as yourab58864 Anti-TLR9 antibody [5G5...

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Thank you for your inquiry.

Unfortunately the epitope for this antibody is not determined.

The following article provides more information regarding the immunogen used: Ahmad-Nejad, P et al; Eur J Immunol 2002, 32: 1958

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Thank you for contacting us.

Unfortunately, all the references and images available are already on the datasheet. We are continuously updating our datasheets, and as soon as we gather further characterisation details about it, we will share i...

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