Overview

  • Product name
    Anti-TLR9 antibody [5G5] (FITC)
    See all TLR9 primary antibodies
  • Description
    Mouse monoclonal [5G5] to TLR9 (FITC)
  • Host species
    Mouse
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: IHC-P, WB, IHC-Fr, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Dog, Human
  • Immunogen

    Purified fusion protein of extracellular domain of human TLR9 (AA 1-815) and human IgGFc

  • Positive control
    • RAW macrophages stimulated with IFN-gamma

Properties

Applications

Our Abpromise guarantee covers the use of ab58864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50.
WB 1/50. Predicted molecular weight: 115 kDa.
IHC-Fr 1/50.
Flow Cyt 1/10 - 1/50.

ab91362 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/50.

Target

  • Function
    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificity
    Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.
  • Sequence similarities
    Belongs to the Toll-like receptor family.
    Contains 26 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • Cellular localization
    Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 289 antibody
    • CD289 antibody
    • TLR 9 antibody
    • TLR9 antibody
    • TLR9_HUMAN antibody
    • Toll like receptor 9 antibody
    • Toll like receptor 9 isoform A precursor antibody
    • Toll like receptor 9 isoform B antibody
    • Toll-like receptor 9 antibody
    see all

Images

  • THP1 cells were incubated with an IgG2a isotype control (A) or a-TLR9 5G5 mAb (B). Cells (140000) were permeabilized with saponin and stained with 0.4 µg 5G5.

References

This product has been referenced in:
  • Dias AA  et al. DNA Sensing via TLR-9 Constitutes a Major Innate Immunity Pathway Activated during Erythema Nodosum Leprosum. J Immunol 197:1905-13 (2016). Read more (PubMed: 27474073) »
  • Ray A  et al. A novel TLR-9 agonist C792 inhibits plasmacytoid dendritic cell-induced myeloma cell growth and enhance cytotoxicity of bortezomib. Leukemia N/A:N/A (2014). Human . Read more (PubMed: 24476765) »
See all 3 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Answer

Merci pour votre réponse.
Comme convenu j'ai mis en place l'envoi d'une unité de ab134369 en remplacement du ab58864. Le numéro de commande de remplacement gratuit de ce produit est : ******.
Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.
N'hésitez pas à nous contacter lors d'une prochaine occasion.

Read More

Answer

Merci de nous avoir contactés.
Nous sommes désolés d'apprendre que le produit que vous avez reçu ne fonctionne pas comme attendu.
Merci pour le partage de vos résultats et de votre protocole, ces informations sont très importantes pour nous car elles nous permettent d'investiguer la source du problème rencontré avec cet anticorps et de prendre les dispositions nécessaires pour assurer une bonne qualité de nos produits.
Je pense que l'anticorps aurait du fonctionner, j'aimerais donc vous proposer un remboursement, ou un avoir, ou un anticorps de remplacement.
Pour l'anticorps de remplacement, je peux vous proposer un produit alternatif, le ab134369 (www.abcam.com/ab134369). Alternativement je peux vous envoyer un autre tube de ab58864 mais celui-ci sera du même lot que celui que vous avez reçu et qui n'a pas fonctionné.
Merci de m'indiquer la résolution qui vous conviendrait le mieux.

Read More

Answer

Thank you for your inquiry.

The products ab16892 and ab58864 are both from the same clone [5G5] and have the same immunogen: Purified fusion protein of extracellular domain of human TLR9 (AA 1-815) and human IgGFc.The immunogen for both is as described in the paper below:

Ahmad-Nejad, P et al; Bacterial CpG-DNA and lipopolysaccharides activate Toll-like receptors at distinct cellular compartments. Eur J Immunol 2002, 32: 1958

Regarding ab72362, the immunogen is BALB/c mice immunized with Ba/F3 cells that stably expressed human Myc-tagged TLR6. The epitope has not been mapped.
I hope this information helps. Please contact us with any other questions.

Read More

Answer

Thank you very much for your interest in our products.

To our knowledge,none of our TLR6 or TLR9 antibodies havebeen tested in bovine. Since we have over 90,000 products we cannot store multiple vial sizes for testing. Therefore, I can offer a discount off a future purchase if you buyanti-TLR6 and anti-TLR9antibodies now, test them inbovine and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody per antibody tested.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceedand let me know the catalogue number of the product/s youwould like to testin bovine. I will then send thediscount code/s.The codesmust be issued before purchasing the antibodies so please wait for my reply before ordering.

2. Purchasethe antibodyeither by phone, fax, or online (www.abcam.com).

3. Test it in bovine.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even ifthe antibodyturns out to be unsuitable for bovine, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for your reply.

Although the exact amount of FITC in ab58864 and ab81197 has not been determined, for the purposes of your calculations, you can assume that it is 40x greater.

Both ab16892 and ab58864, are the same clone of antibody, but the ab58864 has a FITC label. You could also use ab12121:

https://www.abcam.com/TLR9-antibody-26C593-ab12121.html

If there is anything else I can help you with, please let me know.

Read More

Answer

Thank you for contacting us.

Ab81197 Mouse IgG2a (FITC) - Isotype control is supplied in a500 µl size at 4mg/ml. To correctly use this control you will need to use this at the same concentration as yourab58864 Anti-TLR9 antibody [5G5] (FITC) which is supplied at 0.1mg/ml. You will therefore need 40x less of the isotype control than you use for the TLR9 antibody.

We do carry a number of alternative TLR9 antibodies many of which are guaranteed to work in Flow Cytometry. The entire list may be found at the following link:

https://www.abcam.com/index.html?t=73449&pt=1

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Question
Answer

Thank you for your inquiry.

Unfortunately the epitope for this antibody is not determined.

The following article provides more information regarding the immunogen used: Ahmad-Nejad, P et al; Eur J Immunol 2002, 32: 1958

I hope this information is helpful to you. Please do not hesitate to contact us if you need any additional information or advice.

Read More

Answer

Thank you for contacting us.

Unfortunately, all the references and images available are already on the datasheet. We are continuously updating our datasheets, and as soon as we gather further characterisation details about it, we will share it on the Website.

We also encourage our antibody’s users to send us the images obtained with the antibodies and earn Abpoints in compensation: https://www.abcam.com/abpoints.

However, all of our products are covered by our Abpromise® guarantee, which ensures that you can trust our products, and they should work in the tested species and applications stated on the datasheet, or we will offer a replacement, credit, or refund, if reported within 6 months of purchase.

To read about our Abpromise policy: https://www.abcam.com/index.html?pageconfig=abpromise

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up