Overview

  • Product name

  • Description

    Rabbit polyclonal to TLR9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cat, Monkey
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 26-300 of Human TLR9.

  • Positive control

    • Human Liver tissue; THP1 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab125139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 115 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function

    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificity

    Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.
  • Sequence similarities

    Belongs to the Toll-like receptor family.
    Contains 26 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • Cellular localization

    Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 289 antibody
    • CD289 antibody
    • TLR 9 antibody
    • TLR9 antibody
    • TLR9_HUMAN antibody
    • Toll like receptor 9 antibody
    • Toll like receptor 9 isoform A precursor antibody
    • Toll like receptor 9 isoform B antibody
    • Toll-like receptor 9 antibody
    see all

Images

  • ab125139 at 5µg/ml staining TLR9 in formalin-fixed, paraffin-embedded Human liver tissue by Immunohistochemistry.
  • Anti-TLR9 antibody (ab125139) at 1/1000 dilution + THP1 whole cell lysate at 30 µg

    Predicted band size: 115 kDa



    7.5% SDS PAGE

References

This product has been referenced in:

  • Gao C  et al. TLR9 signaling activation at different stages in colorectal cancer and NF-kappaB expression. Onco Targets Ther 11:5963-5971 (2018). Read more (PubMed: 30271180) »
See 1 Publication for this product

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