Overview

  • Product name
  • Description
    Rabbit polyclonal to TLR9
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-FoFr, ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to 16 amino acids near to the C terminus of the protein (Human).

  • Positive control
    • Mouse spleen cells and cell lysate .

Properties

Applications

Our Abpromise guarantee covers the use of ab37154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 21549006
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 95 kDa.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificity
    Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.
  • Sequence similarities
    Belongs to the Toll-like receptor family.
    Contains 26 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • Cellular localization
    Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 289 antibody
    • CD289 antibody
    • TLR 9 antibody
    • TLR9 antibody
    • TLR9_HUMAN antibody
    • Toll like receptor 9 antibody
    • Toll like receptor 9 isoform A precursor antibody
    • Toll like receptor 9 isoform B antibody
    • Toll-like receptor 9 antibody
    see all

Images

  • Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse spleen tissue labeling TLR9 with Anti-TLR9 antibody (ab37154) at 5μg/ml.
  • Lane 1 : Anti-TLR9 antibody (ab37154) at 0.5 µg/ml
    Lane 2 : Anti-TLR9 antibody (ab37154) at 1 µg/ml

    All lanes : Mouse spleen cell lysate
  • ab37154 at 2 µg/ml staining TLR9 in mouse spleen cells by IHC.
  • Immunofluorescence of TLR9 in Mouse Spleen cells using ab37154 at 10 ug/ml.

References

This product has been referenced in:
  • Walsh SW  et al. Increased expression of toll-like receptors 2 and 9 is associated with reduced DNA methylation in spontaneous preterm labor. J Reprod Immunol 121:35-41 (2017). IHC-P ; Human . Read more (PubMed: 28622534) »
  • Luo Y  et al. Regulation of migration and invasion by Toll-like receptor-9 signaling network in prostate cancer. Oncotarget 6:22564-74 (2015). Read more (PubMed: 26087186) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Rabbit Tissue lysate - whole (lung)
Gel Running Conditions
Reduced Denaturing (Gel Bis-Tris 10%)
Loading amount
35 µg
Specification
lung
Blocking step
BSA as blocking agent for 1 hour(s) and 45 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted May 18 2016

Answer

Thank you for contacting Abcam about ab37154. Please find below the suggested standard IHC-P protocol recommended for this antibody, however you may need to optimize it for your own specific needs. Standard Protocol: BUFFERS Working Citrate Buffer 9mL of 0.1M Citric Acid (10.5g citric acid monohydrate to 500mL DI H2O) 41mL of 0.1M Sodium Citrate (14.7g sodium citrate dehydrate to 500mL DI H2O) 450mL of DI H2O Deparaffinization and Rehydration (Cover staining dishes with a lid in each step) 1. Dip slides in three (3) changes of xylene or a xylene substitute for 3 minutes each. 2. Dip slides in two (2) change of 100% alcohol for 3 minutes each. 3. Dip slides in one (1) change of 95% alcohol for 3 minutes. 4. Dip slides in one (1) change of 70% alcohol for 3 minutes. 5. Rinse slides twice (2x) in DI H2O for 5 minutes. Antigen Retrieval (Microwave Method) 6. Soak slides in 3% H2O2 for 5 minutes. 7. Rinse slides twice (2x) in DI H2O for 5 minutes. 8. Soak the slides in the working citrate buffer and cover with a lid. 9. Microwave until the liquid boils, about 1-5 minutes. 10. Remove from heat and let it stand at room temperature for 20 minutes. Page 2 of 2 11. Wash three (3) times for 5 minutes in DI H2O 12. Remove the liquid (do not touch the tissue!) and use a PAP pen to circle around the tissue. Blocking 13. Apply enough 5% BSA with a transfer pipette to cover the tissues. 14. Incubate the slides overnight at 4°C in a humid chamber. Primary Antibody 15. Dilute the primary antibody to the recommended concentration in 1% BSA/PBS diluent. 16. Remove the BSA, and incubate with primary antibody solution for 1 hour at room temperature. 17. Wash slides three (3) times 5 minutes each on the shaker. Secondary Antibody and Detection 18. Dilute the biotinylated secondary antibody to 1:200 in 1% BSA diluent. 19. Incubate with secondary antibody solution for 30 minutes at room temperature. 20. Wash slides in PBS three (3) times 5 minutes each on the shaker. 21. Add enough streptavidin HRP to cover the tissues. Incubate for 30 minutes at room temperature 22. Wash three (3) times 5 minutes each in PBS on the shaker. 23. Add enough DAB to cover the tissues. Once the cells start turning brown (inexperienced technicians may wish to observe this under a microscope), wash twice (2x) in PBS for 5 minutes each on the shaker. If there is anything else I can help

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