Anti-TLR9 antibody (ab37154)
Key features and details
- Rabbit polyclonal to TLR9
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
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Overview
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Product name
Anti-TLR9 antibody
See all TLR9 primary antibodies -
Description
Rabbit polyclonal to TLR9 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide corresponding to 16 amino acids near to the C terminus of the protein (Human).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Imunoaffinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab37154 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 20 µg/ml.
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| WB | (1) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 95 kDa.
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| IHC-P |
Use at an assay dependent concentration.
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| Notes |
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ICC/IF
Use a concentration of 20 µg/ml. |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 95 kDa. |
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IHC-P
Use at an assay dependent concentration. |
Target
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Function
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
Tissue specificity
Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 26 LRR (leucine-rich) repeats.
Contains 1 TIR domain. -
Cellular localization
Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist. - Information by UniProt
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Database links
- Entrez Gene: 81897 Mouse
- SwissProt: Q9EQU3 Mouse
- Unigene: 44889 Mouse
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Alternative names
- CD 289 antibody
- CD289 antibody
- TLR 9 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR9 antibody (ab37154)Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse spleen tissue labeling TLR9 with Anti-TLR9 antibody (ab37154) at 5μg/ml.
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Western blot - Anti-TLR9 antibody (ab37154)Lane 1 : Anti-TLR9 antibody (ab37154) at 0.5 µg/ml
Lane 2 : Anti-TLR9 antibody (ab37154) at 1 µg/ml
All lanes : Mouse spleen cell lysate -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR9 antibody (ab37154)ab37154 at 2 µg/ml staining TLR9 in mouse spleen cells by IHC. -
Immunocytochemistry/ Immunofluorescence - Anti-TLR9 antibody (ab37154)Immunofluorescence of TLR9 in Mouse Spleen cells using ab37154 at 10 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (18)
ab37154 has been referenced in 18 publications.
- Zeng Y et al. Overexpression of retinoid X receptor beta provides protection against oxidized low-density lipoprotein-induced inflammation via regulating PGC1a-dependent mitochondrial homeostasis in endothelial cells. Biochem Pharmacol 188:114559 (2021). PubMed: 33872571
- Zhang S et al. Hydroxychloroquine inhibiting neutrophil extracellular trap formation alleviates hepatic ischemia/reperfusion injury by blocking TLR9 in mice. Clin Immunol 216:108461 (2020). PubMed: 32437924
- Zhou B et al. Hepatoma cell-intrinsic TLR9 activation induces immune escape through PD-L1 upregulation in hepatocellular carcinoma. Theranostics 10:6530-6543 (2020). PubMed: 32483468
- Ahmed S et al. Nitrative DNA damage in lung epithelial cells exposed to indium nanoparticles and indium ions. Sci Rep 10:10741 (2020). PubMed: 32612147
- Choi MR et al. Chloroquine Treatment Suppresses Mucosal Inflammation in a Mouse Model of Eosinophilic Chronic Rhinosinusitis. Allergy Asthma Immunol Res 12:994-1011 (2020). PubMed: 32935491